摘要
目的采用大孔吸附树脂吸附法同时分离纯化枇杷叶中的熊果酸和科罗索酸,并确定分离纯化工艺。方法采用乙醇浸提法将枇杷叶中的熊果酸和科罗索酸进行提取分离纯化,对15种大孔吸附树脂进行筛选,利用大孔吸附树脂柱层析同时分离纯化枇杷叶中的熊果酸和科罗索酸。结果经过对15种大孔吸附树脂的筛选,HPD722的吸附量最好,因此选择HPD722大孔吸附树脂进行柱分离,并确立了最佳的分离纯化工艺。结论HPD722大孔吸附树脂能够使熊果酸和科罗索酸达到较好的分离纯化,工艺可行。
Objective To use macroporous adsorption resin to isolate and purify ursolic acid and corosolic acid in loquat leaf at the same time and determine the method of isolation and purification. Method This experiment used ethanol-extraction to isolate and purify ursolic acid and corosolic acid in loquat leaf and screened 15 kinds of macroporous adsorption resin. Result After screening 15 kinds of macroporous adsorption resin, the best adsorption was HPD722. The optimum technology was assured. Conclusion HPD722 macroporous adsorption resin can isolate and purify ursolic acid and corosolic acid well. The technology is feasible.
作者
沙坤
姜清华
SHA Kun;JIANG Qinghua(Department of Pharmacy,Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2020年第3期238-242,共5页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省科学技术基金(2015020746)。
关键词
枇杷叶
熊果酸
科罗索酸
分离纯化
工艺
loquat leaf
ursolic acid
corosolic acid
isolation and purification
technology
