基于多数据库分析FAM83C在胃癌中的表达及临床意义

Utilizing databases to study the expression of FAM83C in gastric cancer and its clinical significance

目的胃癌的发生发展机制尚不明确。文中探讨FAM83C在胃癌组织中的表达情况,分析其表达差异与临床病理特征及预后的关系,并进一步探讨FAM83C在胃癌中的突变位点及甲基化情况。方法从TCGA数据库、Oncomine数据库中对FAM83C进行数据挖掘,分析FAM83C在胃癌及其他多类癌种中的表达情况;运用LinkedOmics数据库分析FAM83C表达量与胃癌临床病例特征的关系;运用Kaplan-Meier Plotter数据库分析FAM83C表达量与胃癌患者预后关系;运用cBioportal数据库、MethHC数据库分析FAM83C在胃癌中的突变位点及甲基化情况;运用String数据库分析与FAM83C相互作用的蛋白网络。结果Oncomine数据库中56项有关FAM83C表达差异的研究具有统计学意义(P<0.05),其中46项研究提示FAM83C在多种癌组织中高表达,且FAM83C在胃癌中的高表达具有统计学意义(P=0.000733)。同时,分析TCGA数据库中637个胃癌样本,结果显示FAM83C在各种组织类型的胃癌中均呈现高表达(P<0.05)。FAM83C表达量与患者年龄(P=0.0344)、T分期(P=0.034)的差异具有统计学意义(P<0.05)。FAM83C高表达组生存时间更短,预后更差,差异具有统计学意义(P=0.0071)。FAM83C在胃癌中的突变包括错义突变、框移突变、剪接突变3种类型。胃癌中FAM83C基因启动子区甲基化水平较正常胃组织显著升高(P<0.005)。与FAM83C相互作用的蛋白包括SLCO5A1、AKR7A3、MMP24、EIF6、ARL11等,并可能共同参与细胞功能过程。结论FAM83C在胃癌中呈现高表达;且FAM83C表达水平与胃癌恶性程度及不良预后具有一定相关性,在一定程度上表现为癌基因的特性,有望成为胃癌临床诊疗的新靶点。

Objective As the pathogenesis of gastric cancer remains unclear,this paper aims to investigate the expression of FAM83C in gastric cancer tissues,to analyze the relationship between the expression difference and clinicopathological features and prognosis,and to further explore the mutation sites and methylation of FAM83C in gastric cancer.Methods Data mining of FAM83C was conducted by TCGA database and Oncomine database to analyze the expression of FAM83C in gastric cancer and other multiple types of cancer.The relationship between the expression of FAM83C and the clinicopathological characteristics of gastric cancer was analyzed by LinkedOmics database.The effect of FAM83C expression on the prognosis of patients with gastric cancer was analyzed by Kaplan Meier Plotter database.The mutation sites and methylation of FAM83C in gastric cancer was analyzed by cBioportal database and MethHC database.The protein network interacting with FAM83C was analyzed by String database.Results There were 56 studies on FAM83C expression differences in the Oncomine database with statistical significance(P<0.05),among which 46 studies suggested that FAM83C was highly expressed in a variety of cancer tissues,and its high expression in gastric cancer was statistically significant(P=0.000733).Meanwhile,the analysis of 637 gastric cancer samples in the TCGA database showed that FAM83C was highly expressed in all kinds of gastric cancer tissues(P<0.05).The differences between FAM83C expression level and patients'age(P=0.0344)and T stage(P=0.034)were statistically significant(P<0.05).The group with high FAM83C expression had shorter survival time and worse prognosis,and the difference was statistically significant(P=0.0071).FAM83C mutations in gastric cancer include missense mutation,frame-shift mutation and splicing mutation.The methylation level of FAM83C gene promoter region in gastric cancer was significantly higher than that in normal gastric tissue(P<0.005).The proteins interacting with FAM83C include SLCO5A1,AKR7A3,MMP24,EIF6 and ARL11,etc.,and may participate in the cellular function process together.Conclusion FAM83C is highly expressed in gastric cancer.In addition,its expression level has a certain correlation with the degree of malignancy and poor prognosis of gastric cancer,which is manifested in the characteristics of proto-oncogenes to a certain extent,and is expected to become a new target for clinical diagnosis and treatment of gastric cancer.