液相色谱-同位素比质谱技术发展及应用研究进展

Advances in development and application of liquid chromatography coupled with isotope ratio mass spectrometry

液相色谱-同位素比质谱(LC-IRMS)是一种特征化合物同位素分析技术,该技术利用LC IsoLink接口设备实现液相色谱与同位素比质谱的联用,通过检测目标物质的稳定碳同位素比(δ13C),实现样品的产地来源与品质真实性鉴定。该文总结了IRMS与LC-IRMS技术的概况,以及过去20年LC-IRMS的发展历程;归纳整理了LC-IRMS在食品安全、生态与环境、生命科学及考古学等领域的应用情况;评述了LC-IRMS面临的技术局限、挑战及其未来的发展趋势。

The first commercial interface for hyphenating liquid chromatography with isotope ratio mass spectrometry(LC-IRMS)was not available until 2004,and it was commercialized under the name LC IsoLink by Thermo Finnigan.LC-IRMS,a method of compound-specific isotope analysis,has enabled the detection of stable carbon isotope ratios(δ13C)in targeted substances in order to determine their origin and authenticity.This paper provides an overview of IRMS and LC-IRMS techniques,the history and detailed classification of the latter over the past twenty years.The application of LC-IRMS to the fields of food safety,environment and ecology,life sciences,and archeology are reviewed.Finally,the technical limitations,current challenges,and future development trends of LC-IRMS are outlined.