石蒜碱及其与阿苯达唑联合体外抗细粒棘球蚴原头节的作用研究

Study on the action of lycorine and lycorine combined with albendazole against Echinococcus granulosus protoscoleces in vitro

目的观察比较石蒜碱、阿苯达唑及石蒜碱联合阿苯达唑体外抗细粒棘球蚴原头节的作用。方法从感染羊肝上提取细粒棘球蚴原头节,体外培养3d后,分别用石蒜碱(100、500、1000μmol/L)、阿苯达唑(50μmol/L)、石蒜碱联合阿苯达唑(100μmol/L+50μmol/L)处理细粒棘球蚴原头节,在处理后的第1、3、5、7、9d,用1%伊红染色后显微镜下观察原头节的活性,使用多因素重复测量方差分析检验各组间原头节活性率的差异。结果经100、500、1000μmol/L的石蒜碱体外作用9d后,原头节的活性分别为72.70%、14.09%和0。石蒜碱不同剂量组处理后的原头节的活性与对照组比较,差异有统计学意义(P<0.05);石蒜碱高、中、低剂量组之间比较,差异有统计学意义(P<0.05);在相同浓度石蒜碱处理组内,不同测量时间点之间比较,差异具有统计学意义(P<0.05)。石蒜碱联合阿苯达唑(100μmol/L+50μmol/L)、阿苯达唑(50μmol/L)及低剂量石蒜碱(100μmol/L)体外作用9d后,原头节的活性分别为50.20%、90.22%和72.70%,差异有统计学意义(P<0.05)。结论石蒜碱具有体外抗细粒棘球蚴原头节的作用,并表现出时间和浓度依赖性,且与阿苯达唑具有协同作用。

Objective To examine the action of lycorine against Echinococcus granulosus protoscoleces(PSCs)by comparing the efficacy of lycorine,lycorine combined with albendazole,and albendazole in vitro.Methods PSCs were collected from liver hydatid cysts from sheep and cultured for three days in vitro.PSCs were then treated with lycorine(100μmol/L,500μmol/L,1000μmol/L),lycorine combined with albendazole(100μmol/L+50μmol/L),or albendazole(50μmol/L).The viability of protoscoleces was determined with eosin staining(1%)on day 1,day 3,day 5,day7,and day 9.The viability of PSCs was statistically analyzed using two-way repeated measures ANOVA.Results 1.The viability of PSCs was 96.82%in the blank control group and 98.61%in the group treated with DMSO;viability did not differ significantly between the two groups(P>0.05).The viability of PSCs was 72.70%after 9 days of exposure to100μmol/L of lycorine,14.09%after exposure to 500μmol/L,and 0%after exposure to 1,000μmol/L.Viability differed significantly between the lycorine and control groups(P<0.05)and among different concentrations(P<0.05).In the groups treated with lycorine,viability differed significantly at different time points(P<0.05).2.The viability of PSCs was 50.20%after 9 days of exposure to lycorine combined with albendazole,90.22%after exposure to albendazole,and 72.70%after exposure to lycorine.Viability differed significantly between the combined treatment group and the lycorine group and the albendazole group(P<0.05).Conclusion 1.Results indicated that lycorine was efficacious against E.granulosus PSCs in vitro and that its effects are dose-and time-dependent.2.Lycorine and albendazole have synergistic action against E.granulosus PSCs in vitro.