百里香乙醇提取液对黄瓜枯萎病的抑菌作用

Bacteriostasis effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt

【目的】揭示百里香乙醇提取液对黄瓜枯萎病病菌的抑制作用。【方法】采用乙醇萃取,利用索氏抽提装置对百里香(Thymus mongolicus Ronn)茎叶鲜样、茎叶干样、鲜花进行提取,然后将提取液稀释不同倍数,并添加到PDA培养基中,进行黄瓜枯萎病病菌的培养试验,同时对黄瓜枯萎病病菌生长情况及抑菌效果进行观测。【结果】黄瓜枯萎病病菌在添加百里香乙醇提取液培养基上的生长速率降低;百里香各部位乙醇提取液对黄瓜枯萎病病菌均具有抑制作用,且抑菌率因部位和稀释浓度不同而不同,在培养144 h后,百里香茎叶鲜样乙醇提取液稀释2倍的处理抑菌率最高,达45.51%,抑菌效果随浓度的增加而增强。【结论】百里香乙醇提取液对黄瓜枯萎病病菌的生长具有抑制作用,且具有开发成黄瓜枯萎病抑菌剂的潜力。

【Objective】To reveal the inhibitory effect of ethanol extracting solution of thyme on pathogen of cucumber fusarium wilt.【Methods】The ethanol extraction method,the use of soxhlet apparatus of thyme(Thymus mongolicmus Ronn)stem and leaf fresh samples,stem and leaf dry samples,flower fresh samples,then diluted extract solution to different concentration,and added to the PDA medium,cucumber blight pathogen cultivation experiment was carried out.At the same time the cucumber fusarium wilt fungus growth situation and the bacteriostatic effects were observed.【Results】The growth rate of pathogen of cucumber fusarium wilt on the medium of ethanol extracting solution with thyme was decreased.The ethanol extracting solution from all parts of thyme had inhibitory effect on the pathogen of cucumber fusarium wilt,and the bacteriostasis rate was different depending on the location and dilution concentration.After 144 h of culture,the bacteriostasis rate of the ethanol extracting solution which was diluted twice of the fresh sample of thyme stem and leaf,was the highest,reaching 45.51%,and the bacteriostasis effect was enhanced with the increase of the concentration.【Conclusion】The ethanol extracting solution of thyme has the inhibitory effect on the growth of the pathogen of cucumber fusarium wilt,and has the potential to develop into the bacteriostasis agent of cucumber fusarium wilt.