野生黑果枸杞试管苗移栽基质的筛选

Screen out the best transplanting medium of test tube seedlings wild Lycium ruthenicum Murray

【目的】筛选野生黑果枸杞试管苗移栽的最适基质,观察其成活水平。【方法】以内蒙古阿拉善盟、青海省格尔木市野生黑果枸杞无菌试管苗为材料,设3个不同成分的混合基质为处理:壤土+蛭石(3∶1)、壤土+营养土(3∶1)、壤土+蛭石+营养土(3∶1∶1),将无菌试管苗移栽入基质,并观察幼苗的长势情况。【结果】在不同基质处理下,2个地区的野生黑果枸杞在壤土+蛭石+营养土(3∶1∶1)基质下株高、叶长、叶片数均表现较好;在移栽成活率方面,阿拉善盟野生黑果枸杞表现为壤土+营养土(3∶1)基质下存活率最高,格尔木市野果生黑枸杞表现为壤土+蛭石+营养土(3∶1∶1)基质下存活率最高,分别为72.92%、72.00%。【结论】本试验条件下,壤土+蛭石+营养土(3∶1∶1)基质是野生黑果枸杞试管苗移栽的最适基质。

【Objective】In order to screen out the optimal matrix for the transplanting of wild Lycium ruthenicum Murray seedlings in vitro,and to observe its survival level.【Methods】Sterile test tube seedlings of wild fructus Lycium ruthenicum from Alxa League of Inner Mongolia and Golmud City of Qinghai Province were used as materials,and three mixed substrates with different components were set as treatment:loam+vermiculite(3∶1),loam+nutritive soil(3∶1),loam+vermiculite+nutritive soil(3∶1∶1).【Results】Sterile test tube seedlings were transplanted into the substrate and the growth of the seedlings was observed.The results showed that the plant height,the leaf length,and the leaf number of wild Lycium ruthenicum Murray in the two regions were better under different substrate treatments in loam+vermiculite+nutritive soil(3∶1∶1).In terms of the survival rate of transplanting,the loam+nutritive soil(3∶1)was the highest survival rate in alashan wild Lycium ruthenicum Murray,and the loam+vermiculite+nutritive soil(3∶1∶1)was the highest survival rate in golmud wild Lycium ruthenicum Murray,which were 72.92%,72.00%,respectively.【Conclusion】Under the conditions of this experiment,loam+vermiculite+nutritive soil(3∶1∶1)is the optimal substrate for the transplantation of wild Lycium ruthenicum seedlings.