伯氏疟原虫对小鼠脾B细胞和自然杀伤细胞及其表面分子的影响

Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules

为探讨伯氏疟原虫对小鼠脾B细胞和自然杀伤(NK)细胞及其表面分子的影响,将10只雌性C57BL/6小鼠随机分为健康对照组和感染组,每组5只。感染组小鼠经尾静脉注射伯氏疟原虫(106个/鼠)进行感染,健康对照组注射等量生理盐水。感染后6 d,取小鼠脾,制备单细胞悬液,采用流式细胞术检测小鼠B细胞和NK细胞的含量及其表面分子CD62L、CXCR3、CD69的表达水平。结果显示,感染组小鼠脾B细胞百分比含量为(79.2±3.6)%,高于健康对照组的(54.3±4.4)%(P<0.01);感染组脾NK细胞百分比为(2.2±0.7)%,低于健康对照组的(4.6±0.8)%(P<0.01)。CD62L在感染组B细胞中的百分比为(77.7±4.4)%,高于健康对照组的(72.8±7.1)%,但二者差异无统计学意义(P>0.05);CD62L在感染组NK细胞中的百分比为(61.9±4.8)%,低于健康对照组的(86.6±4.2)%(P<0.01)。CXCR3在感染组B细胞中的百分比为(4.1±0.1)%,高于健康对照组的(3.0±0.2)%(P<0.01);CXCR3在感染组NK细胞中的百分比为(2.1±0.9)%,低于健康对照组的(2.3±0.4)%,但二者差异无统计学意义(P>0.05)。CD69在感染组B细胞和NK细胞中的百分比分别为(54.3±4.7)%、(22.2±1.6)%,均高于健康对照组的(1.9±0.4)%、(1.3±0.3)%(P<0.01)。提示感染疟原虫的小鼠可能通过增加B细胞的数量以及上调其表面分子CXCR3和CD69及NK细胞表面分子CD69的表达水平发挥抗疟作用。

To investigate the affects of Plasmodium berghei infection on spleen B cells and NK cells as well as their surface molecules in mice,10 female C57 BL/6 mice were randomly assigned into control group and infection group,five mice each.The mice in the infection group were infected with P.berghei by injection through the tail vein(106 parasites/mouse),while the control group received the same volume of saline.Six days after infection,spleens were collected to prepare single cell suspensions,and using flow cytometry to assay the amount of spleen B cells and NK cells and the expression of their surface molecules CD62 L,CXCR3 and CD69.The results showed that the percentage of B cells in the infection group was(79.2±3.6)%,significantly higher than that in the control group[(54.3±4.4)%,P<0.01].The amount percentage of spleen NK cells in the infection group was(2.2±0.7)%,significantly lower than that in the normal control group[(4.6±0.8)%,P<0.01].The percentage of CD62 L expression on B cells in the infection group was(77.7±4.4)%,higher than that in the normal group(72.8±7.1)%,but with no statistical significance(P>0.05).The percentage of CD62 L expression on NK cells in the infection group was(61.9±4.8)%,which was significantly lower than that in the control group[(86.6±4.2)%,P<0.01].The percentage of CXCR3 expression on B cells in the infection group was(4.1±0.1)%,which was significantly higher than that in the control group[(3.0±0.2)%,P<0.01].The percentage of CXCR3 expression on NK cells was(2.1±0.9)%,which was lower than that of the control group[(2.3±0.4)%],but with no statistical significance(P>0.05).The percentages of CD69 expression on B cells and NK cells were(54.3±4.7)%and(22.2±1.6)%,respectively,both significantly higher than those in the control group[(1.9±0.4)%,(1.3±0.3)%,P<0.01].These results suggest that the mice infected with P.berghei may exert the affect against the infection through increasing B cell recruitment and up-regulating the expression of their surface molecules CXCR3 and CD69,and NK cell surface molecule CD69.