摘要
目的:优化天麻钩藤饮多糖的提取与分离纯化工艺。方法:采用单因素试验优化天麻钩藤饮多糖的提取与分离纯化。结果:天麻钩藤饮多糖最优提取条件为以1∶20的料液比提取4次,每次40min;酶法去除蛋白质的最佳工艺为以0.8%的酶用量,pH=7,在50℃下酶解40min;SephadexG-100柱层析法分离得到9种不同组分。结论:该实验通过对天麻钩藤饮多糖提取及分离纯化的条件优化,为天麻钩藤饮及天麻钩藤饮多糖的开发利用提供了依据。
Objective:Optimization of extraction and separation purificationtechnology of polysaccharides from Tianma gouteng yin.Methods:Optimization of extraction,isolation and purification of polysaccharides from Tianma gouteng yin by single factor test.Results:The optimal extraction conditions of polysaccharides from Tianma gouteng yin are extracting four times with a material-liquid ratio of 1∶20,each time 40 min;the best process to remove protein by enzymatic method is to use 0.8%enzyme dosage,pH is 7,50℃and hydrolyze 40 min;SephadexG-100 column chromatography was used to fractionate 9 different components.Conclusion:In this experiment,the conditions for the extraction and purification of polysaccharides from Tianma gouteng yin were optimized to provide a basis for the development and utilization of Tianma gouteng yin and polysaccharides from Tianma gouteng yin.
作者
张鑫
安加文
王文佳
刘思海
蔡远俊
王珊珊
梁建东
Zhang Xin;An Jiawen;Wang Wenjia;Liu Sihai;Cai Yuanjun;Wang Shanshan;Liang Jiandong(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《亚太传统医药》
2020年第3期43-46,共4页
Asia-Pacific Traditional Medicine
基金
贵州省科技基金项目(黔科合基础[2018]1014)
贵州省千层次人才项目(2014)
贵州省科技基金项目(黔科合J字[2014]2033号)
贵州省普通高等学校工程研究中心项目(黔教合KY字[2015]337)
贵州省国内一流建设学科(中药学)项目(GNYL[2017]008)
贵州省大学生创新创业训练计划重点项目(贵中医大创合字(2015)15号)
贵州省大学生创新创业训练计划一般项目(贵中医大创合字(2015)34号)
贵州省大学生创新创业训练计划一般项目(贵中医时珍大创合字(2015)3号)。
关键词
天麻钩藤饮
多糖
单因素
Tianma Gouteng Yin
Polysaccharides
Single Factor Experiments