C57BL/6小鼠在肥胖建模过程中常见问题及处理对策

Common Problems and Countermeasures of C57BL/6 Mice in the Feeding Process of Diet-Induced Obesity Model

目的发现C57BL/6小鼠在肥胖模型造模过程中出现的问题,并找到解决这些问题的有效方法。方法对出现耳朵感染、脱毛和打架引起外伤的小鼠分别采取相应预防或局部处理措施,使其健康生长。结果打耳标时进行预防性消毒的小鼠耳朵感染风险下降,少量耳朵感染的小鼠经过消毒恢复健康。脱毛小鼠进行隔离消毒后皮毛恢复完整。受轻微外伤小鼠隔离消毒后恢复健康。结论本文提到的C57BL/6小鼠肥胖模型造模过程中出现的各种问题及处理对策可为其他相关科研人员提供参考。

Objective To find out the problems in the process of establishing obesity model of C57BL/6 mice,and find effective ways to solve these problems.Method The mice with ear infection,hair removal and trauma caused by fighting were given corresponding precautions or partial treatments to make them grow healthily.Result Mice that received prophylactic disinfection during ear marking had a reduced risk of ear infection,and a small number of mice with ear infection were restored to health after disinfection.After isolation and disinfection,the fur of depilated mice restored its integrity,and mice with mild trauma restored to health.Conclusion The problems and countermeasures mentioned in this paper can provide references for other relevant researchers in the process of establishing obesity model in C57BL/6 mice.