免疫组化阳性对照组织的选择

Choice of immunohistochemical positive control

目的探讨在进行免疫组化质控时如何选择阳性对照组织。方法选择取材标本中良性扁桃体、阑尾组织和肿瘤旁正常的肝脏、肾脏、乳腺、结肠组织,切成0.3 cm×0.3 cm×0.4 cm大小的组织块,经过脱水、浸蜡、包埋等组织处理后制成阳性对照组织蜡块。将阳性对照块进行连续切片,厚度为4μm,进行CK5/6、P63、CD34、ER等40种抗体的免疫组化染色,对染色结果进行分析。结果40种抗体中扁桃体30种阳性,阑尾32种阳性,肝脏26种阳性,肾脏19种阳性,乳腺21种阳性,结肠28种阳性。结论扁桃体适合做标记鳞状上皮、淋巴系统以及血管和淋巴管相关抗体的阳性对照,阑尾适合做标记腺上皮、平滑肌组织、神经内分泌系统、血管和淋巴管相关抗体的阳性对照,其他正常组织分别适合做同类组织肿瘤标本的阳性对照。

Objective To explore how to select positive control tissue for immunohistochemical quality control.Methods Normal tissues adjacent to the tumor from the liver,kidney,breast,and colon,benign tonsil tissues,and appendiceal tissues were selected and cut into 0.3 cm×0.3 cm×0.4 cm for each block.After dealing with the dehydration,paraffin and embedding,the tissues were made into some paraffin blocks.After serial section,these paraffin blocks were cut into positive section with thickness of 4 microns.Sections were stained by 40 antibodies including CK5/6,P63,CD34 and ER,and the staining results were analyzed.Results Among the 40 antibodies,30 were positive for tonsil,32 for appendix,26 for liver,19 for kidney,21 for breast,and 28 for colon.Conclusion Tonsil is suitable for marking the squamous epithelium lymphatic system and the positive control of vascular and lymphatic related antibodies,appendix is suitable for marking the positive control of vascular and lymphatic related antibodies of the neuroendocrine system of glandular epithelial smooth muscle tissue,and other normal tissues are suitable for marking the positive control of tumor specimens of the same kind of tissues.