基于BGP、TGF-β1的表达探究蛇床子素对去卵巢致骨质疏松大鼠的作用

Study on the Effect of Osthole on Ovariectomized Osteoporotic Rats Based on the Expression of BGP and TGF-β1

目的观察蛇床子素对去卵巢致骨质疏松大鼠的影响。方法选取3月龄雌性SD大鼠30只,随机分为蛇床子素组(A组)、模型对照组(B组)、假手术组(C组),各10只。A、B 2组摘除卵巢构建去势大鼠骨质疏松模型,C组仅进行手术、不摘除卵巢。造模成功后,分别给予相应药物灌胃,连续给药12周,处死。然后测量骨密度,检测治疗后血清BGP、TGF-β1、钙指标。结果B组大鼠股骨骨密度较C组明显降低(P<0.05);经12周治疗,A组股骨骨密度较B组明显升高(P<0.05)。与C组比较,B组大鼠血清中BGP含量升高、TGF-β1降低(P<0.05);与B组比较,A组大鼠血清中BGP降低、TGF-β1含量升高(P<0.05)。与C组比较,B组大鼠血清Ca水平明显降低(P<0.05);与B组比较,A组大鼠血清Ca水平明显升高(P<0.05)。差异均有统计学意义。结论蛇床子素能够有效通过调节大鼠体内激素分泌改善去卵巢大鼠的骨代谢异常,提高骨密度,对骨质疏松症起到一定的防治作用。

Objective To observe the effect of Osthole on ovariectomized osteoporosis rats.Methods Thirty 3-month-old female SD rats were randomly divided into Osthole group(group A),model control group(group B)and sham operation group(group C),with 10 rats in each group.Ovariectomy was performed in group A and group B to establish osteoporosis model in ovariectomized rats.In group C,ovariectomy was performed without ovariectomy.After successful modeling,the rats were given corresponding drugs by gavage for 12 weeks,and then executed.Bone mineral density(BMD)was measured and serum BGP,TGF-β1 and calcium were measured after treatment.Results Bone mineral density of femur in group B was significantly lower than that in group C(P<0.05).After 12 weeks of treatment,bone mineral density of femur in group A was significantly higher than that in group B(P<0.05).Compared with group C,serum BGP and TGF-β1 levels in group B were higher and lower(P<0.05).Compared with group B,serum BGP and TGF-β1 levels in group A were lower and higher(P<0.05).Compared with group C,serum Ca level in group B was significantly lower(P<0.05),while that in group A was significantly higher(P<0.05).The differences were statistically significant.Conclusion Osthole can effectively improve the abnormal bone metabolism and bone mineral density of ovariectomized rats by regulating hormone secretion in vivo,and play a role in preventing and treating osteoporosis.