利用重力场流分离技术探究5种药用淀粉酶解特性

Enzymatic Hydrolysis Characteristics of Five Medicinal Starches by Gravitational Field-Flow Fractionation

目的应用重力场流分离(GrFFF)等技术对5种药用淀粉的酶解特性进行探究。方法首先,应用GrFFF表征5种药用淀粉的酶解速率,并测定还原糖含量验证GrFFF的结果。其次,分别利用扫描电镜(SEM)和粒度仪表征淀粉酶解过程中颗粒形态与尺寸分布的变化。结果5种药用淀粉——蜡质玉米淀粉、马铃薯淀粉、豌豆淀粉、小麦淀粉、普通玉米淀粉的酶解速率依次递减;前2种与后3种分别在6~12 H、12~24 H达到最大酶解速率;随着酶解时间增加,颗粒。尺寸分布范围变广、平均尺寸增加;还原糖含量测定实验、SEM、粒度仪表征结果与GrFFF基本一致。结论GrFFF是表征药用淀粉酶解的有效方法,拓展了GrFFF的应用范围,同时为淀粉在医药与食品领域的开发利用提供参考。

OBJECTIVE To investigate the enzymatic hydrolysis characteristics of five medicinal starches,i.e.,waxy corn starch,potato starch,pea starch,wheat starch and common corn starch,using gravitational field flow fractionation(GrFFF)and other technologies.METHODS Firstly,the enzymatic hydrolysis rates of the five medicinal starches were characterized by GrFFF,and the results of GrFFF were verified by determination of the reducing sugar.Secondly,scanning electron microscopy(SEM)and particle size analyzer were used to characterize the changes of particle morphology and size distribution during amylase hydrolysis.RESULTS The experiments showed that the enzymatic hydrolysis rates of the five medicinal starches were successively decreased.The former two as well as the latter three reached the maximum enzymatic rate at 6-12 h and 12-24 h,respectively.As the enzymatic hydrolysis time increased,the particle size distribution range became wider and the average size increased.The results of reducing sugar quantitation,SEM and particle size analysis were almost identical to those of GrFFF.CONCLUSION This study proves that GrFFF is an effective analytical technology to characterize the enzymatic hydrolysis of medicinal starch,which expands the application range of GrFFF and provides useful reference for the application of starch in medicine and food sciences.