猪A型塞内卡病毒感染诱导猪肾细胞(PK-15)长链非编码RNA差异表达谱的分析

Analysis of differential expression profile of long non-coding RNA in porcine

为研究猪A型塞内卡病毒(SVA)感染猪肾细胞(PK-15)后对于PK-15 lncRNA表达谱的影响,本研究进行了高通量测序以检测SVA感染诱导PK-15产生的差异表达lncRNA。结果显示,在SVA感染后24 h,共诱导1043个lncRNA差异表达,其中已知的lncRNA 420个,未知的lncRNA 623个。GO富集和KEGG通路分析显示,lncRNA靶基因富集于p53信号通路、RIG-I样受体信号通路、MAPK信号通路、IgA生成的肠道免疫网络通路等。根据高通量测序结果选择了8个差异表达的lncRNA采用荧光定量PCR验证,验证结果与高通量测序结果一致。本研究初步表明lncRNA参与了SVA对PK-15细胞的感染过程,为SVA的分子机制研究提供了新的方向。

In order to determine the effect of Senecavirus A(SVA)on the long non-coding RNA(lncRNA)expression in porcine kidney epithelial(PK-15)cells,high-throughput sequencing was performed to detect the differential expression of lncRNAs in SVA-infected PK-15 cells.The results showed that a total of 1043 lncRNAs were differentially expressed at 24 hours after SVA infection,including 420 known lncRNAs and 623 unknown lncRNAs.GO enrichment and KEGG pathway analysis showed that lncRNA target genes were enriched in p53 signaling pathway,RIG-I-like receptor signaling pathway,MAPK signaling pathway,and IgA-generated intestinal immune network pathway.According to the results of high-throughput sequencing,eight differentially expressed lncRNAs were selected for qPCR validation,and the results were consistent with the sequencing result.This study initially indicated that lncRNA was involved in the process of SVA infection in PK-15 cells,providing a new direction for the study of the molecular mechanism of SVA infection.