玫瑰黄链霉菌Men-myco-93-63诱抗粗蛋白对黄瓜的诱抗作用

Induced Resistance of Crude Protein Produced by Streptomyces roseoflavus Men-myco-93-63 to Cucumber Disease

玫瑰黄链霉菌Strempomyces roseoflavus Men-myco-93-63是分离自马铃薯疮痂病自然衰退土壤中的一株拮抗链霉菌,该菌株及其代谢产物对多种重要的植物病原菌都具有较强的抑制作用,为了探明该生防菌产生的诱抗粗蛋白对黄瓜抗病性的诱导作用,采用离体叶片接种的方法,发现诱抗粗蛋白诱导黄瓜叶片灰霉病发病直径显著小于对照;经组织染色法和紫外分光光度计法测定,发现诱抗粗蛋白可以诱导黄瓜叶片中活性氧(ROS)的积累和超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)等抗病相关酶活性的显著提高;通过实时荧光定量PCR(qRT-PCR)测定,发现诱抗粗蛋白还可以诱导黄瓜叶片中PR-1a、PR-3、PR-9和NPR1等抗病相关基因表达的上调,试验结果表明Men-myco-93-63产生的诱抗粗蛋白能够诱导黄瓜抗病性的提升。

Strempomyces roseoflavus Men-myco-93-63 is an antagonistic Streptomyces sp.isolated from the soil where potato scab could decline naturally.The strain and its metabolites have strong inhibitory effects on many important plant pathogens.In order to investigate the induction resistance of the crude protein produced by Men-myco-93-63 to cucumber disease,leaf inoculation was used in vitro.The results showed that the lesion diameter of gray mold in the leaves of cucumber induced by crude protein was significantly smaller than that of buffer solution.Detected by tissue staining and ultraviolet spectrophotometry,the accumulation of reactive oxygen species(ROS)and activity of resistance-related enzymes such as superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO),were significantly increased in cucumber leaves induced by crude protein compared with buffer solution.Quantitative Real-time PCR(qRT-PCR)showed that the crude protein could also induce the up-regulation of the expression of resistance-related genes such as PR-1a,PR-3,PR-9 and NPR1 in cucumber leaves,indicating that the crude protein produced by Men-myco-93-63 could induce disease resistance in cucumber.