麦长管蚜电压门控钠离子通道基因克隆及序列分析

Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae(Fabricius)

克隆获得麦长管蚜电压门控钠离子通道基因cDNA序列,明确其典型特征,为研究麦长管蚜抗性分子机理奠定基础。采用RT-PCR技术,克隆麦长管蚜钠离子通道基因cDNA序列,利用DNASTAR Lasergene 7.10软件对其序列进行分析。克隆得到两条cDNA序列SaNav1和SaNav2(GenBank登录号分别为MN176137和MN161584),SaNav1序列长3423 bp,共编码1141个氨基酸;SaNav2序列长2874 bp,共编码958个氨基酸。同源比对发现,SaNav1与禾谷缢管蚜和桃蚜的第一部分钠离子通道基因相似度分别高达97.81%和97.91%;SaNav2与禾谷缢管蚜和桃蚜的第二部分钠离子通道基因相似性高达97.90%和96.43%。所克隆序列包含4个同源结构域,每个结构域6个跨膜片段(S1~S6),存在钠通道选择性关键残基“DENS”。本文成功克隆了麦长管蚜中钠离子通道基因,为麦长管蚜钠离子通道抗性机制的研究提供依据。

The cDNA sequences of voltage-gated sodium channel gene of Sitobion avenae were cloned and its typical characteristics were identified,which laid a foundation for further studies of the molecular mechanism of pesticide resistance in S.avenae.The sequences were cloned by RT-PCR and analyzed by DNASTAR Lasergene 7.10 software.Two cDNA sequences were obtained,SaNav1 and SaNav2,with GenBank accession numbers of MN176137 and MN161584,respecitvely.The SaNav1 sequence was 3423 bp,encoding a total of 1141 amino acids.The SaNav2 sequence was 2874 bp,encoding a total of 958 amino acids.The homology alignment found that the similarity of SaNav1 and the part I sodium channel genes of Rhodolospi maidis(XP_026806613)and Myzus persicae(XP_022174241)were as high as 97.81%and 97.91%;the similarity of SaNav2 and the part II sodium channel genes of Rhodolospi maidis(ALB75309.1)and Myzus persicae(XP_022174237.1)were as high as 97.90%and 96.43%,respectively.The cloned sequences contain four homologous domains,each with six transmembrane segments(S1—S6)and a sodium channel-selective key residue“DENS”.The sodium channel genes cloned in S.avenae will provide a basis for studying the role of sodium channel in resistance mechanism in S.avenae.