头穴丛刺结合丰富环境对孤独症大鼠行为及IL-10、IL-18的影响

Effects of cluster needling at the scalp points combined with enriched environmental treatment on behavior,IL-10 and IL-18 in autistic rats

目的:观察头穴丛刺结合丰富环境治疗对孤独症大鼠行为及脑内额叶区域白细胞介素(IL)-10、IL-18蛋白表达的影响。方法:通过丙戊酸钠(VPA)建立Wister大鼠孤独症动物实验模型。实验共分为5组:实验组40只孤独症大鼠随机均分为头穴丛刺结合丰富环境组、丰富环境组、头穴丛刺组、模型组,对照组为正常大鼠(n=10)。大鼠21日龄时进行干预,3周后对5组大鼠进行行为学分析、Nissl染色额叶神经元计数及Western Blot检测IL-10和IL-18蛋白表达。结果:与模型组比较,各治疗组异常行为得到改善(P<0.05),额叶神经元存活数量增加(P<0.05),额叶IL-10蛋白表达升高、IL-18蛋白表达降低(P<0.05),且头穴丛刺结合丰富环境组优于丰富环境组或头穴丛刺组(P<0.05)。结论:头穴丛刺结合丰富环境训练可改善孤独症大鼠的异常行为,且优于单纯的丰富环境训练或头穴丛刺治疗,其原因可能是通过抑制脑内额叶区域促炎因子IL-18、促进抑炎因子IL-10蛋白表达对介导孤独症大鼠异常行为的机制产生抑制而实现。

Objective:To observe the effects of cluster needling at the scalp points combined with enriched environmental treatment on the behavior of autistic rats and the protein expression of IL-10 and IL-18 in the frontal lobe of the brain.Methods:Animal models of Wister rat autism was established by valproic acid(VPA).The experiment is divided into 5 groups:In the experimental group,40 autistic rats are randomly divided into cluster needling at the scalp points combined with enriched environment group,enriched environment group,cluster needling at the scalp points group and model group(n=10),the control group are normal rats(n=10).Intervention on the 21st day after birth,after 3 weeks,behavioral analysis,Nissl staining of frontal neurons and Western Blot were used to detect IL-10 and IL-18 protein expression in 5 groups of rats.Results:Compared with the model group,the abnormal behavior of the three after-treatmental groups were improved(P<0.05),the number of surviving neurons in the frontal lobe increased(P<0.05),the exp ression of IL-10 protein in the frontal lobe increased and the expression of IL-18 protein decreased(P<0.05).The cluster needling at the scalp points combined with the enriched environment group is superior to the enriched environment group or the cluster needling at the scalp points group(P<0.05).Conclusion:The cluster needling at the scalp points combined with enriched environmental training can improve the abnormal behavior of autistic rats,and is superior to pure environmental or cluster needling at the scalp points training.The reason may be that inhibition of the proinflammatory factor IL-18 in the frontal lobe and the promotion of the anti-inflammatory factor IL-10 protein expression inhibits the mechanism of abnormal behavior in autistic rats.