RP-HPLC法测定不同产地草龙中3种三萜类成分的含量

Determination analysis of three triterpenses constituents in different habitats of Ludwigia hyssopifoLia(G.Don)Exell by RP-HPLC

目的:对草龙Ludwigia hyssopifoLia(G.Don)Exell药材的质量控制进行研究,为草龙质量标准的制定提供科学依据。方法:采用HP L C法定量测定其中2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇的含量,以COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)为色谱柱;流动相:乙腈(B)-水(A),梯度洗脱(0~18min、29%B;18~45min、53%B);体积流量:1mL/min;检测波长:210nm;柱温:30℃。结果:2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇分别0.0499~0.7982μg(r=0.9995)、0.2000~3.1997μg(r=0.9999)、0.5001~8.0019μg(r=0.9997)线性关系良好,平均回收率分别为100.43%(RSD=1.43%)、100.03%(RSD=1.24%)、100.04%(RSD=1.47%)。结论:该方法准确、可靠、重复性好,可用于草龙药材的质量控制。

Objective:To study the quality control of Ludwigia hyssopifoLia(G.Don)Exell,and to provide scientific basis for the formulation of the quality standards.Methods:The content of 2α,3β,19α,6β,24-pentahydroxylurs-12-ene-18-ursolic acid,2α,3β,19α,23-tetrahydroxyurs-12-ene-28-ursolic acid and ufactol were determined by HPLC.COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)was used as the column;mobile phase:acetonitrile(B)-water(A),gradient elute(0~18min,29%B;18~45min,53%B),the flow rate was 1mL/min,the detection wavelength was 210nm and the temperature at 30℃.Results:2α,3β,19α,6β,24-pentahydroxylurs-12-ene-18-ursolic acid,2α,3β,19α,23-tetrahydroxyurs-12-ene-28-ursolic acid and ufactol have good linear relationships in the range of 0.0499~0.7982μg(r=0.9995),0.2000~3.1997μg(r=0.9999)and 0.5001~8.0019μg(r=0.9997)respectively.And the average recoveries were 100.43%,100.03%and 100.04%;RSD were 1.43%,1.24%,1.47%,respectively.Conclusion:The method is accurate,reliable and repeatable,and can be used for the quality control of Ludwigia hyssopifoLia(G.Don)Exell.