宿主细胞粘附相关基因表达与鸡传染性喉气管炎病毒组织嗜性相关性的研究

Correlation between the expression of adhesion related genes in host cel and the tissue tropism of infectious laryngotracheitis virus infection

为探究宿主细胞粘附相关基因与鸡传染性喉气管炎病毒(ILTV)感染组织嗜性的相关性,本研究采用ILTV特异性定量PCR方法检测了ILTV NP-3毒株感染雏鸡后ILTV在不同组织中的分布情况,并通过RT-qPCR方法在转录水平上检测了攻毒后不同组织中细胞粘附相关基因CDH11、NEGR1和VCAN的转录水平。结果显示,ILTV感染雏鸡后在喉头、气管、哈德氏腺及骨髓中病毒载量相对较高,而在法氏囊、脾脏、胸腺和肝脏中呈阴性。同时,ILTV感染显著促进了细胞粘附相关基因CDH11、NEGR1和VCAN在组织中的表达,且与ILTV在相应组织中的分布呈显著相关性(P<0.05),表明宿主细胞粘附活性对ILTV组织嗜性具有重要作用,具体机制有待进一步研究。本研究探索了ILTV感染组织嗜性与细胞粘附分子间的关系,以期为新型免疫制剂的研制奠定了基础。

To investigate the correlation between the host cell adhesion-related genes and the tissue tropism of laryngotracheitis virus(ILTV) infection, the distribution of ILTV in different tissues of chicken infected with ILTV NP-3 strain was detected by ILTV-specific quantitative PCR, and the transcription level of cell adhesion related genes like CDH11, NEGR1 and VCAN in different tissues were detected by RT-qPCR. The results showed that ILTV infected chickens had relatively high viral load in larynx,trachea, Hardt’s gland and bone marrow, but was negative in bursa, spleen, thymus and liver. At the same time, the expression of cell adhesion related genes CDH11, NEGR1 and VCAN in tissues was significantly promoted by ILTV infection, which was significantly correlated with the distribution of ILTV in the corresponding tissues(P<0.05). These results suggests that the host cell adhesion activity plays an important role in the tissue tropism of ILTV, but the specific mechanism needs further study. This study explored the relationship between tissue tropism and cell adhesion molecules in ILTV infection, and provided a theoretical basis for the development of new immunologics.