黑胸散白蚁肠道内芽孢杆菌F8产纤维素酶发酵工艺研究

Study on Cellulase Fermentation Process of Bacillus F8 in Black-Breasted Termite Intestine

对从黑胸散白蚁肠道内筛选出来的一株产纤维素酶的芽孢杆菌F8产酶发酵工艺条件进行了优化。从菌株培养基的碳源、氮源、装液量,培养时间、温度、初始pH、接种量等条件进行单因子优化,并在优化的基础上,采用正交试验优化。该菌株产纤维素酶的最佳发酵条件为:羧甲基纤维素钠质量百分比为0.5%,氮源质量百分比为1.5%,装液量40 mL/300mL,初始pH 7.0,接种量5%,温度35℃,时间2 d。

The fermentation conditions of cellulase producing bacillus F8 from a strain of termite blackbreast were optimized.Single factor optimization was carried out from the carbon source,nitrogen source,loading amount,culture time,temperature,initial pH,inoculum.Based on the optimization,orthogonal test is used.The best fermentation conditions for the strain to produce cellulase were as follows:sodium carboxymethyl cellulose mass percentage was 0.5%,nitrogen source mass percentage was 1.5%,liquid content was 40 mL/300ml,initial pH 7.0,inoculum amount was 5%,temperature 35℃,time 2 d.