基于UHPLC-Q-TOF/MS技术对扶正化瘀胶囊入血成分的快速鉴别

Rapid identification of chemical constituents in serum and plasma of Fuzheng Huayu capsules by UHPLC-TOF/MS

目的本研究运用超高效液相色谱-高分辨飞行时间质谱(UHPLC-Q-TOF/MS)联用技术鉴别扶正化瘀胶囊入血成分。方法色谱分离采用ACQUITY UPLCHSS T3(2.1 mm×100 mm,1.8μm);流动相0.1%甲酸水溶液(A)?0.1%甲酸乙腈溶液(B),梯度洗脱条件:0~3 min,2%B;3~18 min,2%~50%B;18~22 min,50%~95%B;22~25 min,95%B。平衡时间为10 min,流速为0.4 ml/min,分析时间为25 min。质谱定性电喷雾离子源采用在正、负离子模式扫描,采集范围从100~1100 m/z。结果在血清样品中一次性鉴别出49个成分,其中正、负离子模式下均有响应的有4个。结论本研究较全面地阐明了扶正化瘀胶囊的入血成分,丰富了扶正化瘀胶囊的科学内涵,为该复方的深入研究奠定了基础。

Objective To identify the blood components of Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time-of-flight mass spectrometry(UHPLC-Q-TOF/MS).Methods ACQUITY UPLCHSS T3(2.1 mm×100 mm,1.8μm)was used to chromatographic separation;mobile phase was 0.1%formic acid aqueous solution(A)?0.1%formic acid acetonitrile solution(B).The gradient elution conditions included:0?3 min,2%B;3?18 min,2%?50%B;18?22 min,50%?95%B;22?25 min,95%B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min.The mass spectrometry was characterized by electrospray ionization by a positive-negative ion mode scan with a range of 100-1100 m/z.Results 49 components were identified in the serum samples at one time,of which 4 were positive and negative ion modes.Conclusion The blood components of Fuzheng Huayu capsule were clarified by this method,which enriched the scientific connotation of Fuzheng Huayu capsule,and laid the foundation for the in-depth study of the compound.