血清一氧化氮、血管内皮生长因子水平与喉癌患者颈部淋巴结转移的关系

The relationship between serum nitric oxide,vascular endothelial growth factor levels and cervical lymph node metastasis in patients with laryngeal cancer

目的探讨血清一氧化氮(NO)、血管内皮生长因子(VEGF)水平与喉癌患者颈部淋巴结转移的关系。方法选择39例喉癌患者、34例喉部良性病变患者及36例健康体检者,分别作为喉癌组、喉部良性病变组及健康对照组。检测3组研究对象的血清NO、VEGF水平,采用Spearman相关分析法分析血清NO、VEGF水平与喉癌患者颈部淋巴结转移的相关性,采用受试者工作特征(ROC)曲线分析NO、VEGF单独及联合检测对喉癌患者颈部淋巴结转移的预测价值。结果喉癌组患者的血清NO、VEGF水平均高于喉部良性病变组和健康对照组(P﹤0.05),喉部良性病变组患者的血清NO、VEGF水平均高于健康对照组(P﹤0.05)。39例喉癌患者中,14例患者发生颈部淋巴结转移,25例患者未发生颈部淋巴结转移,颈部淋巴结转移患者的血清NO、VEGF水平均高于颈部淋巴结未转移患者(P﹤0.05)。血清NO、VEGF水平均与喉癌患者颈部淋巴结转移呈正相关(r=0.399、0.385,P﹤0.05)。NO、VEGF单独及联合检测预测喉癌患者颈部淋巴结转移的曲线下面积(AUC)分别为0.740、0.731和0.814。结论喉癌患者的血清NO、VEGF水平较高,且其在颈部淋巴结转移患者中的水平高于无颈部淋巴结转移的患者,两项指标联合检测对喉癌患者颈部淋巴结转移具有较好的预测价值。

Objective To analyze the relationship between nitric oxide(NO),vascular endothelial growth factor(VEGF) and cervical lymph node metastasis in patients with laryngeal cancer.Method Thirty-nine patients with laryngeal cancer,34 cases with benign laryngeal lesions,and 36 healthy controls were included in the analysis as laryngeal cancer group,benign laryngeal lesions group,and healthy control group,respectively.The levels of serum NO and VEGF were measured in the three groups,and the correlation between serum NO and VEGF and cervical lymph node metastasis in patients with laryngeal cancer was investigated by Spearman correlation analysis,besides,the receiver operating characteristic(ROC) curve was used to analyze the predictive value of NO or VEGF alone or in combination for lymph node metastasis in laryngeal cancer patients.Result The levels of serum NO and VEGF in laryngeal cancer group were significantly higher than those in benign laryngeal lesions group and healthy control group(P<0.05),and were significantly higher in patients with benign laryngeal lesions than in healthy controls(P<0.05).Among the 39 cases with laryngeal cancer,cervical lymph node metastasis were identified in 14 cases while not in another 25 cases;the levels of serum NO and VEGF in patients with cervical lymph node metastasis were significantly higher than those in the ones without cervical lymph node metastasis(P<0.05).The levels of serum NO and VEGF were positively correlated with cervical lymph node metastasis in laryngeal cancer patients(r=0.399,0.385,P<0.05).The area under the curve(AUC) of NO or VEGF applied alone or in combination in predicting cervical lymph node metastasis of laryngeal cancer were 0.740,0.731 and0.814,respectively.Conclusion The levels of serum NO and VEGF are relatively high in laryngeal cancer patients,and are augmented in patients with cervical lymph node metastasis compared to those without cervical lymph node metastasis,besides,the combined detection of the two indicators demonstrates a good predictive value in determining cervical ly mph no de metastasis in laryngeal cancer.