葡萄籽原花青素对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响

Effects of Grape Seed Procyanidins on Growth Performance,Semen Quality,Antioxidant Indexes in Testis and Epididymis of Ram

本试验旨在研究饲喂葡萄籽原花青素(GSPs)对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响。试验选取4月龄、体重[(22.75±1.20)kg]相近的杜泊×小尾寒羊杂交一代公羔48只,随机分为4组,每组12只羊,在基础饲粮饲喂基础上分别补饲0(对照组)、10(10GSPs组)、20(20GSPs组)、40 mg/kg BW(40GSPs组)的GSPs。试验期共60 d,其中预试期15 d,正试期45 d。结果表明:1)10GSPs和20GSPs组平均日增重和平均日采食量显著高于对照组和40GSPs组(P<0.05),40GSPs组与对照组差异不显著(P>0.05)。补饲GSPs对料重比影响不显著(P>0.05)。2)GSPs补饲组的附睾重量均显著高于对照组(P<0.05),睾丸、附睾指数在4组间无显著差异(P>0.05)。20GSPs和40GSPs组的顶体完整率显著高于10GSPs组和对照组(P<0.05),精子畸形率显著低于对照组(P<0.05)。3)20GSPs和40GSPs组睾丸、附睾组织中的总抗氧化能力(T AOC)、谷胱甘肽过氧化物酶(GSH Px)活性显著高于对照组(P<0.05),GSPs补饲组附睾超氧化物歧化酶(SOD)活性均显著高于对照组(P<0.05),20GSPs和40GSPs组睾丸、附睾组织中丙二醛(MDA)含量显著低于10GSPs和对照组(P<0.05)。4)睾丸组织中,20GSPs组的SOD mRNA相对表达量显著高于10GSPs组和对照组(P<0.05),40GSPs组的谷胱甘肽过氧化物酶4(GPx4)mRNA相对表达量显著高于10GSPs组和对照组(P<0.05),4组间的过氧化氢酶(CAT)和核因子E2相关因子2(Nrf2)mRNA相对表达量差异不显著(P>0.05);附睾组织中,20GSPs和40GSPs组SOD mRNA相对表达量显著高于对照组(P<0.05),与10GSPs组差异不显著(P>0.05);GSPs补饲组GPx4 mRNA相对表达量均显著高于对照组(P<0.05);20GSPs和40GSPs组Nrf2 mRNA相对表达量显著高于对照组(P<0.05)。综上,补饲适量GSPs可以促进绵羊公羔生长,提高睾丸和附睾组织抗氧化能力,进而改善精液品质;在本试验条件下GSPs最适补饲量为20 mg/kg BW。

The aim of this experiment was to investigate the effects of feeding grape seed procyanidins(GSPs)on growth performance,semen quality,testis and epididymis antioxidant indexes of male lambs.A total of for ty eight 4 month old Dorper()×small Tail Han sheep(♀)F1 male lambs with similarly weight of(22.75±1.2)kg were randomly selected and divided into 4 groups equally.All the lambs fed a basal diet and supple mented with 0(control group),10(10GSPs group),20(20GSPs group)and 40 mg/kg BW(40GSPs group)GSPs,respectively.The experiment was conducted over 60 d,with the first 15 d for adaptation and 45 d for test.The results showed as follows:1)average daily gain(ADG)and average daily feed intake(AD FI)in 10GSPs和20GSPs groups were significantly higher than those in the control group and 40GSPs group(P<0.05),with no significant difference between 40GSPs group and control group(P>0.05).No significant difference was observed in feed conversion ratio among GSPs groups(P>0.05).2)The weight of epididymis in the GSPs groups was significantly higher than that in the control group(P<0.05),and the indexes of testis and epididymis had no significant difference among the four groups(P>0.05).The acrosome integrity in 20GSPs and 40GSPs groups was significantly higher than that in 10GSPs and control groups(P<0.05),and the rate of sperm malformation in 20GSPs and 40GSPs groups was significantly lower than that in control group(P<0.05).3)The activity of total antioxidant capacity(T AOC)and glutathion peroxidase(GSH Px)in testis and epididymis in 20GSPs and 40GSPs groups were significantly higher than those in control group(P<0.05).The superoxide dismutase(SOD)activity in epididymis in three GSPs groups was significantly higher than that in the contol group(P<0.05),and the malonaldehyde(MDA)content in the testis and epididymis in 20GSPs and 40GSPs groups was significantly lower than that in 10GSPs group and control group(P<0.05).4)In testis,the relative expression levels of SOD mRNA in 20GSPs group and glutathione peroxidase 4(GPx4)mRNA in 40GSPs group were significantly higher than those in 10GSPs group and control group(P<0.05).There was no significant difference among four groups in the relative expression levels of catalase(CAT)and nuclear factor E2 correlation factor 2(Nrf2)mRNA(P>0.05).In epididymis,the relative ex pression levels of SOD mRNA in 20GSPs and 40GSPs groups was significantly higher than that in control group(P<0.05),but had no significantly difference compared with 10GSPs group(P>0.05).The relative expression level of GPx4 mRNA in three GSPs groups was significantly higher than that in control group(P<0.05).Additionally,the relative expression level of Nrf2 mRNA in 20GSPs and 40GSPs groups was signifi cantly higher than that in control group(P<0.05).In summary,dietary supplementation of GSPs can promote the growth and improve the antioxidant status of testis and epididymis of ram,thereby improving semen quali ty.The optimal GSPs supplemental level is 20 mg/kg BW under the condition of this experiment.