一测多评法同时测定九味沉香胶囊中9种成分

Simultaneous determination of nine constituents in Jiuwei Chenxiang Capsules by QAMS

目的 建立一测多评法同时测定九味沉香胶囊(沉香、广枣、黄芪等)中沉香四醇、毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、木香羟内酯、去氢木香内酯、肉豆蔻木脂素、去氢二异丁香酚的含有量.方法 该药物甲醇提取液的分析采用Agilent Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm);流动相[乙腈-甲醇(9∶1)]-0.1%甲酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长225、254、270 nm.以木香羟内酯为内标,计算其他8种成分的相对校正因子,测定含有量.结果 9种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.95%~ 100.12%,RSD 0.73%~ 1.51%.一测多评法所得结果与外标法接近.结论 该方法简便准确,重复性好,可用于九味沉香胶囊的质量控制.

AIM To establish a quantitative analysis of multi components by single marker(QAMS)method for the simultaneous determination of agarotetrol,calycosin7 O β D glucopyranoside,ononin,calycosin,formonon etin,costunolide,dehydrocostus lactone,myrislignan and dehydrodiisoeugenol in Jiuwei Chenxiang Capsules(Aquilariae lignum Resinatum,Choerospondiatis Fructus,Astragali Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30℃thermostatic Agilent Zorbax SB C18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of[acetonitrile methanol(9∶1)]-0.1%formic acid flow ing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 225,254,270 nm.Costunolide was used as an internal standard to calculate the relative correction factors of the other eight constitu ents,after which the content determination was made.RESULTS Nine constituents showed good linear relation ships within their own ranges(r>0.9990),whose average recoveries were 96.95% 100.12%with the RSDs of 0.73%-1.51%.CONCLUSION This simple,accurate and reproducible method can be used for the quality con trol of Jiuwei Chenxiang Capsules.