芩百清肺浓缩丸对肺炎支原体感染的影响

Effects of Qinbai Qingfei Pills on Mycoplasma pneumoniae infection

目的探讨芩百清肺浓缩丸对肺炎支原体感染的影响。方法采用MTT、流式细胞术考察体外芩百含药血清对肺炎支原体感染的A549细胞增殖能力和凋亡的影响。运用RT-PCR、免疫组织化学检测体内小鼠肺组织MEK、c-jun mRNA及蛋白表达,HE染色观察肺组织病理改变。结果与模型组比较,芩百含药血清(10%、20%)组能促进肺炎支原体感染的A549细胞增殖并抑制细胞凋亡(P<0.05)。体内实验表明,芩百清肺浓缩丸能减轻MP感染小鼠肺组织损伤;促进肺组织MEK、c-jun mRNA及蛋白表达(P<0.05)。结论芩百通过激活MEK和c-jun因子表达促进肺炎支原体感染肺上皮细胞的修复。

AIM To investigate the effects of Qinbai Qingfei Pills(QQPs)on Mycoplasma pneumoniae(MP)infection.METHODS MTT and flow cytometry were used to investigate the effects of QQPs serum on the prolif eration and apoptosis of A549 cells with MP infection in vitro.Mice were subjected to the pulmonary determinations of expression of MEK and c jun mRNA and protein by RT PCR and immunohistochemistry,and pathological changes by HE staining in vivo.RESULTS In contrast to the model group,QQPs serum groups(10%,20%)demonstrated promoted proliferation of A549 cells infected with MP and inhibited cell apoptosis(P<0.05).In vivo experiments showed that concentrated QQPs reduced lung tissue damage of MP mice,and promoted pulmonary expression of MEK and c jun mRNA and protein(P<0.05).CONCLUSION QQPs can promote the repair of pulmonary epithelial cells with MP infection by activating the expression of MEK and c jun factors.