基于LC-MS/MS法分析生、炙甘草中水溶性成分

Analysis of water soluble components in raw and honey roasted licorice based on LC MS/MS

目的 基于LC-MS/MS定性/定量分析生、炙甘草中水溶性成分.方法 采用LC-MS/MS正、负离子模式对生、炙甘草水溶性成分进行检测,并以Marker View软件分析甘草蜜炙前后的差异性成分;在此基础上采用梯度波长HPLC法对生、炙甘草8种主要差异性成分(4种黄酮及4种三萜皂苷)进行定量分析比较.结果 从中鉴定出30种水溶性差异性成分,其中包括12种黄酮类和18种皂苷类;其中黄酮类异甘草苷、芹糖异甘草苷在蜜炙后平均含有量分别上升5.11%、25.94%,甘草苷、芹糖甘草苷含有量分别下降21.77%、18.43%,而4种三萜皂苷平均含有量在蜜炙后均出现下降,幅度在10.07%~16.69%.结论 该方法准确稳定,重复性好,可为炙甘草的炮制及质量评价研究提供一定的参考.

AIM To analyze the water soluble components in raw and honey roasted licorice based on LC MS/MS.METHODS The water soluble components of raw and honey roasted licorice were detected by LC MS/MS negative and positive ion modes,and the differential components before and after the processed licorice were ana lyzed by Marker View software;on this basis,eight major components(four flavonoids and four triterpene sapo nins)of raw and processed licorice were quantitatively analyzed and compared by gradient wavelength.RESULTS Thirty water soluble components in raw and processed licorice were identified,including twelve fla vonoids and eighteen saponins.The average contents of flavonoids isoliquiritin and isoliquiritin apioside increased by 5.11%,25.94%,respectively,and the contents of liquiritin and liquiritin apioside decreased by 21.77%,18.43%,respectively,while the average contents of four triterpenoid saponins decreased from 10.07%to 16.69%.CONCLUSION This accurate,stable and reproducible method can be used for the processing and quality evaluation of licorice.