高通量测序法分析感杨树灰斑病前后叶围微生物多样性

Leaf Microbial Diversity before and after Phyllosticta populea Sacc by High-throughput Sequencing

采用高通量测序法,探究小黑杨(Populus simonii×P.nigra)灰斑病(Coryneum populinum Bres.)病叶与健康叶之间共生细菌和真菌群落结构的差异。结果表明:小黑杨叶片感病前后细菌与真菌的OTU变化巨大,2组样品共有的细菌种类有27个,仅占细菌总数的5.49%,共有真菌种类有22个,仅占真菌总数的0.71%。2组样品中菌落结构基本相同,但相对丰度差异较大,在门水平下,变形菌门(Proteobacteria)是优势细菌,子囊菌门(Ascomycota)是优势真菌。在属水平下,鞘氨醇单胞菌属(Sphingomonas)是健康叶中的优势细菌,假单胞菌属(Pseudomonas)是病叶中的优势细菌;枝孢属(Cladosporium)是健康叶中的优势真菌,镰刀菌属(Fusarium)是病叶中的优势真菌,其次是棒盘孢属(Coryneum)。

The high-throughput sequencing was used to investigate the differences in symbiotic bacterial and fungal community structures between Phyllosticta populea Sacc leaf spot disease and healthy leaves.The bacterial and fungal OTUs of P.populea leaves changed greatly before and after susceptibility to disease.The two samples shared 27 bacterial species,accounting for only 5.49%of the total bacteria,and 22 fungal species,accounting for only 0.71%.Two substantially identical sample colonies structures,but large differences in relative abundance.At the phylum level,Proteobacteria is the dominant bacterium,and Ascomycota is the dominant fungus.At the genus level,Sphingomonas is the dominant bacteria in healthy leaves,and Pseudomonas is the dominant bacteria in diseased leaves;Cladosporium is the predominant fungus in healthy leaves,Fusarium is the predominant fungus in diseased leaves,followed by Coryneum.