人脑胶质瘤MAGE-D4和CDC25A的表达及其相关性

Expression and correlation of MAGE-D4 and CDC25A in human glioma

目的:研究黑色素瘤相关抗原基因-D4(MAGE-D4)和细胞分裂周期蛋白25A(CDC25A)在人脑胶质瘤(胶质瘤)中的表达水平和临床意义,并探究二者的关系。方法:利用GEPIA在线数据库分析胶质瘤组织中MAGE-D4和CDC25A的表达情况,然后收集47例胶质瘤组织,逆转录实时荧光定量聚合酶链反应(PT-qPCR)验证MAGE-D4和CDC25A的表达,并进一步统计分析其与临床病理指标的关系及二者表达相关性。结果:GEPIA数据库显示MAGE-D4与CDC25A在胶质瘤组织中均高表达(P<0.05);PT-qPCR结果显示所检测的胶质瘤组织MAGE-D4 mRNA和CDC25A mRNA的表达水平均明显高于正常脑组织,且二者表达呈明显正相关关系(r=0.3174,P<0.05);此外,MAGE-D4 mRNA的表达与WHO分级和Ki-67表达相关,而CDC25A mRNA表达仅与WHO分级相关(P<0.05)。结论:CDC25A与MAGE-D4在胶质瘤组织中呈正相关关系,提示二者可能共同在胶质瘤的发生发展中发挥重要作用,可能成为胶质瘤生物治疗的潜在靶点。

Objective: To study the expression level and clinical significance of melanoma associated antigen geneD4(MAGE-D4)and mitotic cyclin 25 A(CDC25 A)in human gliomas,and to explore their relationship.Methods:The expression of MAGE-D4 and CDC25 A in glioma tissues were analyzed by GEPIA online database.Then 47 glioma tissues were collected,and the expression of MAGE-D4 and CDC25 A were verified by reversr transcriptase real-time fluirescent polymerase chain reaction(PT-qPCR).The correlation of MAGE-D4,CDC25 A,and clinical pathological indexes were analyzed.Results: GEPIA database showed that both MAGE-D4 and CDC25 A were highly expressed in glioma tissues(P<0.05).qPCR showed that the expression levels of MAGE-D4 mRNA and CDC25 A mRNA in glioma tissues were significantly higher than those in normal brain tissues,and there was a significant positive correlation between MAGE-D4 mRNA and CDC25 A mRNA expression(r=0.3174,P<0.05).In addition,the expression of MAGE-D4 mRNA was correlated with WHO grade and Ki-67 expression,while the expression of CDC25 A mRNA was only correlated with WHO grade(P<0.05).Conclusion: There is a positive correlation between CDC25 A and MAGE-D4 in glioma tissue,which suggests that both of them may play an important role in the occurrence and development of glioma and may become potential targets for glioma biotherapy.