高效液相一测多评法同时测定参苏片中葛根素、迷迭香酸、白花前胡甲素、白花前胡乙素、白花前胡E素的含量

Simultaneous Determination of Puerarin,Rosmarinic Acid,Praeruptorin A,Praeruptorin B and Praeruptorin E in Shensu Tablets by HPLC-QAMS

目的:建立高效液相一测多评法同时测定参苏片中葛根素、迷迭香酸、白花前胡甲素、白花前胡乙素、白花前胡E素的含量。方法:采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);以甲醇-0.1%甲酸溶液为流动相,梯度洗脱;流速:0.9 ml·min^-1;检测波长:250 nm(葛根素)、321 nm(迷迭香酸、白花前胡甲素、白花前胡乙素、白花前胡E素);柱温:30℃;以迷迭香酸为内标物,计算葛根素、白花前胡甲素、白花前胡乙素、白花前胡E素的相对校正因子,测定其含有量,同时采用外标法测定这5个组分的含量。结果:5种成分在各自范围内线性关系良好(r≥0.9991),平均加样回收率97.46%~99.83%,RSD为0.71%~1.67%,一测多评法计算值与外标法实测值间无明显差异。结论:利用相对校正因子对参苏片中5种成分的含量测定是可行的,所建立的高效液相一测多评法可以用于参苏片的质量评价研究。

Objective:To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous determination of puerarin,rosmarinic acid,praeruptorin A,praeruptorin B and praeruptorin E in Shensu tablets.Methods:The analysis was performed on a Kromasil C18 column(250 mm×4.6 mm,5μm)with gradient elution,and the mobile phase was comprised of methanol-0.1%formic acid solution at the flow rate of 0.9 ml·min^-1.The detection wavelengths were set at 250 nm(puerarin)and 321 nm(rosmarinic acid,praeruptorin A,praeruptorin B and praeruptorin E).With rosmarinic acid as the internal standard,the relative correction factors of puerarin,rosmarinic acid,praeruptorin A,praeruptorin B and praeruptorin E were calculated,and further constituent content determination was compared by using both the relative correction factors method and the external standard method.Results:The five components showed good linear relationships within different ranges(r≥0.9991),and the average recoveries were between 97.46%and 99.83%with the RSDs of 0.71%-1.67%.The results obtained by QAMS had not significant differences with those obtained by the external standard method.Conclusion:The use of relative correction factors is practicable to determine the contents of five components in Shensu tablets.The HPLC-QAMS method can be used for the quality assessment of Shensu tablets.