樱柏酸对叔丁基过氧化氢诱导SH-SY5Y神经细胞氧化应激损伤的保护作用

Protective effects of communic acid against the TBHP-induced oxidative stress injury in SH-SY5Y nerve cells

采用TBHP处理SH-SY5Y细胞的方法,建立了细胞氧化应激损伤模型,用以评价樱柏酸对细胞氧化应激损伤的保护作用及机制。同时借助MTS法、β-半乳糖苷酶(SA-β-gal)染色法、乳酸脱氢酶(LDH)含量检测法、Annexin V-FITC/PI双染法、2′,7′-二氯荧光素二乙酸盐(DCFH-DA)法以及酶联免疫法(ELISA)法等对细胞的活性及衰老相关指标进行了检测。研究结果显示樱柏酸对TBHP诱导的SH-SY5Y氧化应激损伤细胞模型具有保护作用,并且能够降低细胞衰老比例、细胞凋亡水平,其作用机制可能通过减轻细胞膜和DNA等氧化应激损伤、提高体内抗氧化酶SOD和GSH-Px酶活力减少ROS含量起效。

The aim of this study was to investigate the neuroprotective effects of communic acid against cell oxidative stress injury induced by t-butylhydroperoxide(TBHP) in human neuroblastoma cells and its mechanisms. Meanwhile, MTS method, SA-glycosyl lactate dehydrogenase(LDH) staining method, Annexin v-fitc/PI double dyeing method, 2′,7′-dichlorofluorescein diacetate(dcfh-da) method and Enzyme-linked immunosorbent assay(ELISA) was used to detect the activity and senescence of cells.The results showed that communic acid has a protective effect on TBHP-induced oxidative damage in SH-SY5 Y cells, and can reduce the cell senescence ratio and the apoptosis level. The mechanism may be that TBHP reduced oxidative damage in cell membrane and DNA, and decreased the level of ROS content by improving enzyme activities such as antioxidant enzymes SOD.