基于Planteye F500三维定量研究淹水胁迫下烯效唑对大豆的生长调控

3D quantification on regulatory effects of uniconazole on soybean growth under flooding stress based on Planteye F500

以大豆垦丰14为材料,利用Planteye F500三维扫描仪定量研究烯效唑对淹水胁迫下大豆生长的影响,建立三维表型参数与形态生理指标之间的相关性。结果表明:淹水处理降低了大豆株高、数字生物量、叶片倾斜度、植株3D叶面积、投影叶面积和光穿透深度等形态指标,分别较对照下降20.51%、45.25%、15.60%、31.22%、18.67%和26.59%。喷施烯效唑降低了淹水处理下大豆数字生物量、株高、3D叶面积、投影叶面积以及光穿透深度,并较淹水处理低22.50%、19.25%、5.94%、14.80%和56.12%。大豆植株实际叶面积、3D叶面积、投影叶面积三者呈正相关关系。通过部分生理指标相关性分析发现,SPAD值与归一化植被指数NDVI呈正相关,与色调值、光穿透深度呈负相关。相关性分析结果为表型组学与作物栽培学的结合提供了依据。

A quantitative study was conducted by using multispectral 3 D scanner PlantEye F500 to understand the effect of uniconazole on soybean flooding stress. Established correlations between phenotypic parameters and morphological and physiological indicators helped to promote the development of phenomics. The tested soybean material in this study was Kenfeng 14. Results indicated that flooding stress decreased soybean height, digital biomass, leaf inclination, 3 D leaf area, projected leaf area and light penetration depth, and which were reduced by20.51%, 45.25%, 15.60%, 31.22%, 18.67% and 26.59% compared to CK. Spraying uniconazole under flooding stress condition also decreased digital biomass, height, 3 D leaf area, projected leaf area and light penetration depth,and which were declined by 22.50%, 19.25%, 5.94%,14.80% and 56.12% compared to flooding stress. Soybean 3 D leaf area and projected leaf area were in positive correlation with actual leaf area. Through the correlation analysis of some physiological parameters, the value of SPAD showed a positive correlation with NDVI, and the negative correlation with hue and light penetration depth. The correlation analysis results provided the basis related to phenomics and crop cultivation.