早期微量喂养早产儿肠道菌群变化特征与喂养不耐受发生的关系研究

Study on the Relationship between the Change Characteristics of Intestinal Microflora and the Incidence of Feeding Intolerance in Early Micro-feeding Premature Infants

目的:探究早期微量喂养早产儿肠道菌群变化特征与喂养不耐受发生的关系。方法:选取笔者所在医院2019年1-6月收治的40例早期微量喂养早产儿,观察组为喂养不耐受早产儿确诊者,对照组为同时期喂养耐受早产儿,以喂养不耐受发生24 h内(T1)和喂养不耐受恢复后(T2)为时间节点,采集各早期微量喂养早产儿的粪便标本,利用PCR技术进行菌群分析,对比其肠道菌群变化情况差异。结果:T1时,观察组在肠道的肺炎克雷伯菌和大肠杆菌水平明显高于对照组,乳酸杆菌和双歧杆菌水平明显低于对照组(P<0.05);T2时,观察组与对照组的上述肠道菌群水平比较差异均无统计学意义(P>0.05)。结论:在早期微量喂养早产儿中,肠道菌群的变化参与喂养不耐受的发生,其中不耐受发生尤其与肺炎克雷伯菌和大肠杆菌水平增加有一定关系。

Objective:To investigate the relationship between the change of intestinal microflora and the incidence of feeding intolerance in early micro-feeding premature infants.Method:This research selected 40 early micro-feeding premature infants admitted to our hospital from January to June 2019.The observation group were infants with feeding intolerance while the control group with feeding tolerance.Within 24 hours of the feeding intolerance onset (T1) and after the recovery of feeding intolerance (T2) as the time node,the stool samples collected from each early micro-feeding premature infants were analyzed by PCR to compare the changes of intestinal microflora.Result:At T1,the numbers of intestinal Klebsiella pneumonia and Escherichia coli in the observation group were significantly higher than that of the control group,but the numbers of Lactobacillus and Bifidobacteria were significantly lower than that of the control group (P<0.05).Moreover,at T2,there were no significant differences in the expression of the above intestinal microflora between the observation group and the control group (P>0.05).Conclusion:In early micro-feeding premature infants,the change of intestinal microflora may contribute to the development of feeding intolerance.And the incidence of feeding in tolerance is particularly associated with the increased level of Klebsiella pneumonia and Escherichia coli.