HOTAIR EZH2和VEGF在鼻咽癌组织中的表达情况及其对预后的影响

Expression of HOTAZR,EZH2 and VEGF in Nasopharyngeal Carcinoma and Their Influence on Prognosis

目的:分析血管内皮生长因子(VEGF)、zeste基因增强子同源物2(EZH2)和长链非编码RNA Hox转录反义RNA(HOTAIR)在鼻咽癌组织中的表达情况及其对预后的影响。方法:采用回顾性研究,收集本院72例鼻咽癌(鼻咽癌组)和54例鼻咽慢性炎症(对照组)患者的临床资料,通过免疫组织化学法检测两组VEGF和EZH2蛋白表达情况,并采用实时荧光定量聚合酶链反应检测患者VEGF、EZH2和HOTAIR mRNA的表达情况。根据HOTAIR在鼻咽癌组织中的表达情况,将鼻咽癌患者分为低表达组与高表达组,比较两组临床病理特征的差异。结果:鼻咽癌组VEGF和EZH2蛋白表达水平均明显高于对照组(P<0.001)。相比对照组,鼻咽癌组VEGF、EZH2和HOTAIR mRNA相对表达量均明显升高(P<0.05)。高表达组病理分级、N分期、M分期、近期疗效与低表达组比较,均存在明显差异(P<0.05);两组年龄、性别、病理类型的比较,均无明显差异(P>0.05)。结论:VEGF、EZH2和HOTAIR高表达于鼻咽癌组织,并且HOTAIR可能通过EZH2和VEGF而起到诱导鼻咽癌远处转移的作用,且与患者预后不良存在密切关系。

Objective:To analyze the expression of vascular endothelial growth factor(VEGF),enhancer of zeste homolog 2(EZH2)and long-chain noncoding RNA Hox transcript antisense RNA(HOTAIR)in nasopharyngeal carcinoma and its effect on prognosis.Methods:The clinical data of 72 patients with nasopharyngeal carcinoma(NPC group)and 54 patients with chronic inflammation of nasopharynx(control group)were collected by retrospective study.The expression of VEGF and EZH2 were detected by immunohistochemistry,and the expression of VEGF,EZH2 and HOTAIR mRNA were detected by real-time fluorescence quantitative polymerase chain reaction.According to the expression of hotair in nasopharyngeal carcinoma,nasopharyngeal carcinoma patients were divided into low expression group and high expression group.Results:The expression of VEGF and EZH2 in nasopharyngeal carcinoma group was significantly higher than that in control group(P<0.001).Compared with the control group,the relative expression of VEGF,EZH2 and HOTAIR mRNA in nasopharyngeal carcinoma group was significantly increased(P<0.05).There were significant differences in pathological grade,N stage,M stage,short-term efficacy between the high-expression group and the low-expression group(P<0.05);there were no significant differences in age,gender,pathological type between the two groups(P>0.05).Conclusion:VEGF,EZH2 and HOTAIR are highly expressed in nasopharyngeal carcinoma,and hotair may play a role in inducing distant metastasis of nasopharyngeal carcinoma through EZH2 and VEGF,which is closely related to poor prognosis.