血管内皮生长因子表达及基因多态性与妊娠期糖尿病风险的相关性

Correlation between Vascular Endothelial Growth Factor Expression andGene Polymorphism and Risk of Gestational Diabetes

目的:探讨血管内皮生长因子(VEGF)表达及基因多态性妊娠期糖尿病(GDM)风险的相关性。方法:选取在2016年2月至2018年2月期间来我院就诊的GDM孕妇76例及同期正常健康妊娠孕妇76例进行研究。其中GDM孕妇作为观察组,正常妊娠孕妇作为对照组。采用免疫吸附试验法测定血清VEGF的水平,采用聚合酶链反应-限制性片段长度多态性检测VEGF-460C/T基因多态性,比较两组患者血清VEGF的水平及VEGF-460C/T基因型的分布情况。结果:观察组孕妇的FPG、糖化血红蛋白及VEGF水平显著高于对照组,但HDL-C水平却显著低于对照组(P<0.05)。两组孕妇在年龄、BMI、新生儿出生体重、TC、TG及LDL-C指标上差异无统计学意义(P>0.05)。两组孕妇的VEGF-460C/T基因型CC、CT、TT及等位基因C、T分布符合Hardy-Weinberg平衡。观察组患者的CC基因型及C等位基因频率(27.63%、51.32%)显著高于对照组(10.53%、34.87%)(P<0.05)。CC、CT、TT型孕妇的GDM发病率分别为72.41(21/29)、49.31%(36/73)及47.50%(19/40),其中CC型孕妇GDM发病率显著高于CT、TT型孕妇(χ^2=4.492、χ^2=4.283,P<0.05)。二元Logistic多因素回归分析结果显示:血清VEGF水平、HDL-C及VEGF基因多态性是孕妇发生GDM独立危险因素。结论:血清VEGF水平及VEGF基因多态性是孕妇发生GDM的独立危险因素,且C等位基因可能是GDM的易感基因。

Objective:To investigate the correlation between vascular endothelial growth factor(VEGF)expression and genetic polymorphism of gestational diabetes mellitus(GDM).Methods:76 patients with GDM pregnant women who came to our hospital during the two-year period from February 2016 to 2018 and 76 normal pregnant women with normal pregnancy were selected for study.Among them,GDM pregnant women were used as observation group,and normal pregnant women were used as control group.The level of serum VEGF was determined by immunosorbent assay.The VEGF-460C/T gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism.The serum VEGF level and the distribution of vegf-460c/T genotype were compared between the two groups.Results:The levels of FPG,glycated hemoglobin and VEGF in the observation group were significantly higher than those in the control group,but the HDL-C level was significantly lower than that in the control group(P<0.05).There were no significant differences in age,BMI,neonatal birth weight,TC,TG and LDL-C between the two groups(P>0.05).The distribution of VEGF-460C/T genotype CC,CT,TT and allele C and T in the two groups of pregnant women accorded with Hardy-Weinberg equilibrium.The CC genotype and C allele frequency(27.63%,51.32%)of the observation group were significantly higher than those of the control group(10.53%,34.87%)(P<0.05).The incidence rates of GDM in pregnant women with CC,CT and TT were 72.41(21/29),49.31%(36/73)and 47.50%(19/40),respectively.The incidence of GDM in CC women was significantly higher than that in CT and TT.Pregnant women(χ^2=4.492,χ^2=4.283,P<0.05).The results of binary logistic multivariate regression analysis showed that serum VEGF levels,HDL-C and VEGF gene polymorphisms were independent risk factors for GDM in pregnant women.Conclusion:In summary,serum VEGF level and VEGF gene polymorphism are independent risk factors for GDM in pregnant women,and C allele may be the susceptible gene of GDM.