免疫组织化学染色技术标记CK(AE1/AE3)在乳腺癌前哨淋巴结冰冻剩余组织中表达的意义

Expressions of CK(AE1/AE3)by immunohistochemical staining in the frozen sentinel lymph nodes of breast cancer

目的探讨免疫组织化学染色CK(AE1/AE3)在乳腺癌患者前哨淋巴结中的表达情况对乳腺癌患者淋巴结转移分析的应用价值。方法收集乳腺癌患者前哨淋巴结组织1585枚,前哨淋巴结冰冻剩余组织经过规范化脱水、透明、石蜡包埋组织块、连续切片,做苏木精-伊红(hematoxylin-eosin staining,HE)染色和免疫组织化学CK(AE1/AE3)标记。结果1585枚前哨淋巴结冰冻剩余组织经免疫组织化学染色CK(AE1/AE3)标记显示,有95枚发生淋巴结宏转移,36枚发生淋巴结微转移,有54枚发生孤立性肿瘤细胞(isolated tumour cell,ITC)转移;根据HE染色结果显示,冰冻报告为淋巴结可疑转移5例,经免疫组织化学染色CK(AE1/AE3)标记显示3例未发生转移。结论在乳腺癌患者前哨淋巴结中进行免疫组织化学染色CK(AE1/AE3)标记,降低HE染色的假阳性率,有助于孤立性肿瘤细胞的检出,提高诊断淋巴结转移的准确率,为临床治疗方案的选取提供依据。

Objective To investigate the value of application of CK(AE1/AE3)by immunohistochemical staining in sentinel lymph node metastasis analysis of breast cancer patients.Methods A total of 1585 sentinel lymph node tissues of breast cancer were collected.The frozen residual tissues of sentinel lymph nodes were subjected to standardized dehydration,transparent,paraffin-embedded and sectioned.Hematoxylin-eosin staining(HE)and immunohistochemical CK(AE1/AE3)markers were used to compare the difference of the two methods in sentinel lymph node metastasis screening.Results Immunohistochemical staining of 1585 sentinel lymph node tissues showed that there were 95 lymph node macrometastases,36 lymph node micrometastasis,and 54 isolated tumour cells(ITC);The results of HE staining showed that the frozen report was 5 cases of suspicious lymph node metastasis.But immunohistochemical staining of CK(AE1/AE3)showed that there was no metastasis in 3 cases.Conclusion Immunohistochemical staining of CK(AE1/AE3)in sentinel lymph nodes of breast cancer patients can reduce the false positive rate of HE staining,help to detect solitary tumor cells,improve the accuracy of lymph node metastasis,thus effectively assist pathological diagnosis,and provide a basis for the selection of clinical treatment.