基于氢键作用的金纳米粒子比色法检测尿酸

Determination of Uric Acid by Gold Nanoparticles Colorimetry Based on Hydrogen Bonding

尿酸含量高可使人产生痛风等疾病,尿酸的测定是临床检测重要的生化指标之一。金纳米粒子比色法检测尿酸实验联系实际生活,将科研前沿和教学内容有机结合起来,可以激发学生的学习兴趣,加深学生对经典理论的理解,增加学生对科研前沿的了解。本实验利用金纳米粒子吸光系数高的特点,通过尿酸与三聚氰胺反应后,抑制三聚氰胺诱导的金纳米粒子聚集,从而达到检测尿酸的目的。随着溶液中尿酸浓度的增加,溶液颜色由蓝变红,差别明显,视觉效果好,容易分辨。

High content of uric acid can cause gout and other diseases,and thus uric acid is one of the important biochemical indicators in clinical test.Detection of uric acid based on gold nanoparticle colorimetry combines the forefront of scientific research with the content of teaching,which can stimulate students’interest in learning,deepen their understanding of classical theories and increase their understanding of the forefront of scientific research.In this experiment,gold nanoparticles were used to detect uric acid by inhibiting the accumulation of gold nanoparticles induced by melamine after the reaction of uric acid with melamine.With the increase of the concentration of uric acid in the solution,the color of the solution changes from blue to red,and the difference is obvious.The visual effect is good and easy to observe.