NLRP3对匹罗卡品致癫痫大鼠模型海马齿状回的作用

Effect of NLRP3 on Hippocampal Dentate Gyrus in Pilocarpine-Induced Rat Epilepsy Model

目的:探讨NLRP3在匹罗卡品致癫痫大鼠模型中海马齿状回的表达及作用。方法:大鼠64只,随机选取12只为对照组,其余大鼠建立氯化锂-匹罗卡品癫痫模型,造模成功后随机分为1 d、7 d、14 d、28 d组,每组12只。ELISA检测大鼠血清IL-1β、TNF含量;免疫组化、RT-PCR检测大鼠海马组织齿状回NLRP3、Caspase-1在癫痫造模后不同时间点的表达水平。结果:7 d、14 d、28 d组血清中IL-1β、TNF的含量较对照组高(P<0.01),且28 d组最高,高于其他组(P<0.01);7 d、14 d、28 d组大鼠海马组织齿状回NLRP3、Caspase-1平均光密度,海马组织NLRP3、Caspase-1 mRNA表达均高于对照组(P<0.01),且28 d组最高,高于其他组(P<0.01)。结论:在慢性自发性癫痫形成过程中,NLRP3、Caspase-1在大鼠模型海马齿状回中表达升高,可能与激活炎症反应有关。

Objective:To explore the effect of NLRP3 on the hippocampal dentate gyrus in the pilocarpine-induced rat epilepsy model.Methods:Twelve rats were randomly selected from 64 adult SD rats as controls,and the others were induced by lithium-pilocarpine to create the epilepsy model.After model establishment,the rats were randomly divided into the 1 d,7 d,14 d,and 28 d groups(n=12 per group).The serum levels of IL-1βand TNF were detected with ELISA.The expression of NLRP3 and Caspase-1 in the hippocampal dentate gyrus was measured with immunohistochemistry and RT-PCR.Results:The serum levels of IL-1βand TNF in the 7 d,14 d,and 28 d group rats were higher than those in control group rats(P<0.01),and that of the 28 d group was highest among all groups(P<0.01).The average optical density and mRNA expression of NLRP3 and Caspase-1 in the hippocampal dentate gyrus of 7 d,14 d,and 28 d group rats were higher than those in control group rats(P<0.01),and that of the 28 d group was highest among all groups(P<0.01).Conclusion:Expression of NLRP3 and Caspase-1 increased in the hippocampal dentate gyrus of pilocarpine-induced model rats during the progression of chronic spontaneous epilepsy.This may be associated with activation of the inflammatory response.