LncRNA9606对猪流行性腹泻病毒复制的影响

LncRNA9606 regulates porcine epidemic diarrhea virus replication in LLC-PK1 cells

长链非编码RNA(lncRNA)是一种长度大于200 nt的非编码RNA分子,在调节多种生命活动中起着重要作用。本团队前期研究发现,宿主的lncRNA表达谱在猪流行性腹泻病毒(PEDV)感染后发生显著变化。本研究选择其中一条差异表达的LncRNA TCONS_00009606(LncRNA9606),以研究它对PEDV复制的影响。为此,本研究首先检测LncRNA9606在PEDV感染后不同时间点的动态表达变化。结果,PEDV感染显著上调LncRNA9606的表达。随后对猪肠上皮细胞、派伊尔氏淋巴集合结以及外周血单个核细胞中LncRNA9606的表达丰度进行了分析,结果显示派伊尔氏淋巴集合结和外周血单个核细胞中的LncRNA9606含量显著高于小肠上皮细胞系细胞,并主要定位于细胞核中。在LLC-PK1细胞中过表达LncRNA9606后,PEDV复制水平显著降低。由此可见,LncRNA9606可在LLC-PK1细胞中抑制PEDV病毒的复制。

Long noncoding RNA(lncRNA)is a type of non-coding RNA molecule longer than 200 nt,which plays vital roles in biological events.Our previous results demonstrated that the host's lncRNA expression profile was significantly changed after porcine epidemic diarrhea virus(PEDV)infection.In this study,one of the lncRNAs,lncRNA9606,was selected to investigate its impact on PEDV replication.First,the kinetics of lncRNA9606 expression in IPEC-J2 cells were examined at different time points after PEDV infection.The results confirmed that PEDV infection significantly upregulated the expression of lncRNA9606.The lncRNA9606 expression levels in different cells or tissues were evaluated and the results showed that the amount of lncRNA9606 in Peyer's patches and peripheral blood mononuclear cells were significantly higher than that in small intestinal epithelial cell lines.It was mainly localized in the nucleus.Further investigations indicated that overexpression of lncRNA in LLC-PK1 cells significantly inhibited PEDV replication.In conclusion,lncRNA9606 can suppress the PEDV replication in LLC-PK1 cells.