摘要
目的:探讨正常宫颈、CIN及宫颈鳞癌组织中P-gp、MRP1、Survivin三种耐药蛋白的相关表达,以及三种耐药蛋白与宫颈鳞癌新辅助化疗的关系。方法:采用免疫组织化学SP法和自动图像分析系统检测三种耐药相关蛋白P-gp、MRP1、Survivin在15例正常宫颈组织、15例CIN以及30例Ⅰb2-Ⅱa期宫颈鳞癌患者新辅助化疗前后中的表达。结果:从正常宫颈组织到CIN再到宫颈鳞癌,P-gp、MRP1、Survivin的阳性表达量显著升高(P<0.01);新辅助化疗有效组P-gp和Survivin阳性表达量显著低于新辅助化疗无效组(P<0.01),且化疗后P-gp的阳性表达量显著高于化疗前水平(P<0.01),Survivin的阳性表达量低于化疗前水平(P<0.05);新辅助化疗有效组MRP1表达量虽然低于无效组,但是差异无统计学意义(P>0.05),且化疗前后的阳性表达量亦无统计学意义(P>0.05)。结论:宫颈鳞癌组织中存在多种耐药相关蛋白的共同表达,联合检测多个耐药相关蛋白,可以在一定程度上更好地指导临床化学用药,选择有效的个体化治疗方案。
Objective:To investigate the expression of P-gp,MRP1 and survivin in normal cervical epithelia,CIN and cervical squamous cell carcinoma,and the relationship between them and Neoadjuvant chemotherapy(NACT).Methods:The expression of P-gp,MRP1 and survivin were determined by immunohistochemical S-P method and colour pathological image computer analysis system,in 15 cases of normal cervical epithelia,15 cases of CIN and 30 cases of invasive squamous cell cervical carcinoma in stageⅠb2-ⅡA before and after NACT.Results:It was found that the positive level of P-gp,MRP1 and survivin expression increased in the order of normal cervical epithelium,CIN and invasive squamous cell carcinoma of cervix(P<0.01).The positive level of P-gp and survivin in the effective group was significantly lower than that in the invalid group(P<0.01),and the expression of P-gp after NACT is significantly higher than before(P<0.01),the expression of survivin after NACT is significantly lower than before(P<0.01).There was no correlation of the positive level of MRP1 between the effective and invalid group after NACT(P>0.05),and there was also no correlation of the expression of MRP1 before and after NACT(P>0.05).Conclusion:Multiple drug resistance proteins are expressed in cervical squamous cell carcinoma,combined detection of them can better guide clinical medication and select individualized treatment options.
作者
陈芬
彭瑞宇
孟佳
张丽萍
CHEN Fen;PENG Ruiyu;MENG Jia(Kunming IVF Hospital,Kunming City,Yunnan Province 650000)
出处
《医学理论与实践》
2020年第10期1571-1574,共4页
The Journal of Medical Theory and Practice