面向中药精制过程的中药水提液淀粉含量测定方法的改进与比较

Determination Method of Starch Content in Chinese Medicine Water Extract:Comparative and Improvement

目的建立快速、准确的中药水提液中的淀粉含量测定方法。方法针对中药水提液体系,对淀粉含量试剂盒方法进行方法学考察,以系统设计的淀粉模拟溶液为研究对象,比较碘显色法、酶水解法、优化后的试剂盒法3种方法的含量测定结果。结果优化后的试剂盒法重现性良好,测试结果稳定。淀粉标准品模拟溶液精密度相对标准偏差RSD(n=6)为1.91%,测得各淀粉模拟溶液与水提液重复性相对标准偏差RSD(n=6)为1.82%~4.32%,平均加样回收率在99.18%~104.41%,RSD(n=9)为2.33%~4.94%。针对中药水提液体系淀粉含量检测的最佳方法为优化后的试剂盒法。结论优化后所得的淀粉测定方法简单、高效,并具有较好的精密度及准确度,可为中药水提液中淀粉含量测定的方法研究提供依据。

OBJECTIVE To establish a rapid and accurate method for determination of starch content in Chinese medicine water extract.METHODS In this study,the starch content assay kit method was optimized for the traditional Chinese medicine water extraction system and the methodology was investigated.Then the starch simulation solution was taken as the research object.The content measurement results of three methods including iodine colorimetry,enzymatic hydrolysis method and the optimized starch content assay kit method were compared.RESULTS The optimized starch content assay kit method has good reproducibility and stable test results.The precision tests showed a relative standard deviation(n=6)of 1.91%.The accuracy test relative standard deviations(n=6)of the starch simulated solution and the aqueous extract were 1.82%~4.32%.The average recovery sampling rate of starch sample were 99.18%~104.41%,and the relative standard deviations(n=9)were 2.33%~4.94%.The best method was the optimized starch content assay kit method.CONCLUSION It shows that the optimized starch content assay kit method has good precision and accuracy,can be used as a detection method for determination of starch content in Chinese medicine water extract.