基于氧化-还原胺化反应改性海藻酸盐制备载药性电纺纳米复合纤维

Preparation of Drug-loaded Electrospun Composite Nanofibers Based onOxidation-reductive Amination Reaction Modified Alginate

以辛胺为疏水改性剂,采用氧化-还原胺化反应制得具有两亲性的海藻酸衍生物(RAOA)。通过FTIR、1H NMR、荧光光谱和光学接触角测量仪对RAOA的结构和性能进行表征。进而,以聚乙烯醇(PVA)为助纺剂,对疏水改性的RAOA进行电纺性能的研究。通过光学接触角测量仪、电导率仪、流变仪和扫描电子显微镜对RAOA/PVA纺丝液的物理性能和与之相应的RAOA/PVA纳米复合纤维的形貌进行了测试,考察了该电纺纳米复合纤维对疏水性布洛芬的负载和释药性能。结果表明,辛胺成功接枝到海藻酸钠(SA)分子链上,RAOA的临界聚集浓度为0.43 g/L,说明RAOA具有良好的两亲性。氧化-还原胺化反应改性RAOA不能从根本上改变单一RAOA溶液的可纺性,但是它可以改善RAOA/PVA纺丝液的电纺性能,提高RAOA在RAOA/PVA电纺纳米复合纤维中的含量。载药后的SA/PVA电纺纳米复合纤维在释药初期有突释行为,但改性后的RAOA可以有效地减缓布洛芬的释放速度,提高其缓释性能。

The amphiphilic alginate derivative(RAOA)was prepared via oxidative-reductive amination using the octylamine as a hydrophobic modifier.The structure and property of RAOA were characterized by FTIR,1H NMR,fluorescence spectrum and optical contact angle measuring instrument.Furthermore,the electrospinning of hydrophobically modified alginate derivative,RAOA,was studied by using PVA as a spinning auxiliary.The physical properties of RAOA/PVA spinning solution and the morphology of corresponding RAOA/PVA electrospun composite nanofibers were determined by optical contact angle measuring instrument,electrical conductivity meter,rheometer and scanning electron microscopy.And the loading and release properties of the electrospun composite nanofibers for hydrophobic ibuprofen were also investigated.Experimental results showed that the octylamine had successfully grafted onto the alginate molecular chains.The critical aggregation concentration measured by fluorescence spectrum was reduced to 0.43 g/L,which demonstrated the good amphipathy of RAOA.The oxidation-reductive amination modification of RAOA could not fundamentally change the spinnability of a single RAOA solution,but it could enhance the content of RAOA in RAOA/PVA electrospun composite nanofibers.The drug-loaded SA/PVA electrospun composite nanofibers would release rapidly at the beginning,but RAOA can effectively retard the drug release rate of ibuprofen,thus improving the sustained release performance of RAOA/PVA electrospun composite nanofibers.