抗Pg-IgY脂质体的制备及理化性质的测定

Preparation and physicochemical properties determination of anti-Pg-IgY liposome

目的通过观察特异性抗牙龈卟啉单胞菌卵黄抗体脂质体(抗Pg-IgY脂质体)包封率和体外缓释效果,探讨临床上辅助治疗慢性牙周炎并提高其疗效的理论依据与新的治疗方法。方法使用薄膜分散法制备抗Pg-IgY脂质体,动态光散射粒度分布仪测定抗Pg-IgY脂质体的Zeta电位和粒径,利用透析法结合酶标仪测定包封率,考察其在0.9%生理盐水中的体外释放率,并利用透射电子显微镜对其进行质量评估。结果脂质体的Zeta电位为(-38.55±0.16)mV,平均粒径为(82.48±2.3)nm,多分散指数为(0.313±0.01),脂质体的包封率为40%,在0.9%生理盐水中4 h内累积释放率<20%,24 h内达到约50%,108 h释放率为79.9%。结论抗Pg-IgY脂质体具有较高的包封率,其粒径属于纳米范围,稳定性好,有望实现在牙周袋内的长循环缓释作用。

Objective To investigate the theoretical basis and new treatment methods of specific anti-Porphyromonas gingivalis yolk antibody liposome(anti-Pg-IgY liposome)in clinical adjuvant treatment of chronic periodontitis and improve its efficacy by observing its entrapment efficiency and the effect of in vitro sustained release.Methods Anti-Pg-IgY liposomes were prepared by thin film dispersion method.Zeta potential and particle size of anti-Pg-IgY liposomes were determined by dynamic light scattering particle size distribution instrument.Entrapment efficiency was determined by dialysis method combined with Elisa.In vitro release rate of anti-Pg-IgY liposomes in 0.9%physiological saline was investigated,and its quality was evaluated by transmission electron microscope.Results The Zeta potential of liposome was(-38.55±0.16)mV,the average particle size was(82.48±2.3)nm,the polydispersity index was(0.313±0.01),the entrapment efficiency of liposome was 40%,the cumulative release rate in 0.9%physiological saline was less than 20%within 4 h,about 50%within 24 h,and the release rate in 108 hours was 79.9%.Conclusion Anti-Pg-IgY liposome has high entrapment efficiency,nanometer size and good stability.It is expected to realize long-cycle sustained release in periodontal pocket.