TRPV1基因多态性与新疆汉族、哈萨克族儿童哮喘相关性研究

Relationship between TRPV1 gene polymorphism and childhood asthma of Kazakh and Han in Xinjiang

目的探讨TRPV1基因rs150846、rs8065080两位点单核苷酸多态性(SNPs)与新疆汉族、哈萨克族儿童哮喘的相关性。方法选取哮喘儿童99例作为病例组,其中哈萨克族52例,汉族47例,另选取77例儿童作为对照组,其中哈萨克族39例,汉族38例。提取静脉血DNA,采用Massarray SNP分型方法检测TRPV1基因的两位点多态性。结果①病例组与对照组在rs150846位点的基因型及等位基因频率分布比较差异均有统计学意义(P<0.05);而rs8065080位点的基因型及等位基因频率分布比较差异均无统计学意义(P>0.05)。②哈萨克族病例组与对照组在rs150846位点的基因型及等位基因频率分布比较,差异均有统计学意义(P<0.05);A等位基因携带者发生哮喘的危险性为G等位基因携带者的1.941倍(95%CI:1.037~3.636);在rs8065080位点基因型及等位基因频率分布比较,差异均无统计学意义(P> 0. 05)。(3)汉族病例组与对照组在rs150846、rs8065080位点的基因型频率分布比较,差异无统计学意义(P> 0. 05)。结论 TRPV1基因的rs150846位点多态性可能与哈萨克族儿童哮喘易感性有关。

Objective To explore the relationship of two single nucleotide polymorphisms(SNPs), rs150846 and rs8065080, of TRPV1 gene with childhood asthma of Kazakh and Han in Xinjiang. Methods The participants enrolled were 99 inpatients and outpatients(case group) who received treatment of Pediatrics, including 52 Kazakhs and 47 Hans. 77 healthy controls(control group) underwent physical examination, including 39 Kazakhs and 38 Hans. All the children were taken 2 ml of peripheral venous blood, and DNA of the blood sample was extracted. The Massarray SNP typing technique was used to detect the polymorphisms of TRPV1 gene in peripheral venous blood cells. Results ① The patterns of genotype and allele frequencies of rs150846 at the TRPV1 locus had statistical difference between the groups(P<0. 05),but the patterns of genotype and allele frequencies of rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05). ② Among the Kazakh population, the patterns of genotype and allele frequencies of rs150846 at the TRPV1 locus had statistical difference between the groups(P<0.05). The allele A of rs150846 had a 1.941-fold(95%CI:1.037~3.636) increased risk of developing asthmatic diseases compared with those who had allele G. But the patterns of genotype and allele frequencies of rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05).③ Among the Han population, the patterns of genotype and allele frequencies of rs150846 and rs8065080 at the TRPV1 locus did not differ significantly between the groups(P>0.05). Conclusion The rs150846 gene polymorphism of TRPV1 gene could be associated with susceptibility to childhood asthma of Kazakh in Xinjiang.