大肠癌组织中的EphA2和Ki-67蛋白与患者手术预后的相关性研究

Correlation of EphA2 and Ki-67 proteins in colorectal cancer tissues with prognosis of patients

目的探讨大肠癌组织中Eph受体酪氨酸激酶A2(Eph receptor tyrosine kinase A2,EphA2)、Ki-67蛋白在大肠癌组织中的表达与患者预后的相关性。方法选取2016年7至12月收治的行手术治疗的大肠癌患者115例,使用免疫组化法分别检测癌组织和癌旁组织中EphA2、Ki-67蛋白表达情况,分析两种蛋白表达与肿瘤大小、癌组织分化、Dukes分期、淋巴结转移的相关性,Kaplan-Meier曲线对比癌组织中EphA2、Ki-67蛋白表达与患者生存时间的关系。结果癌组织中EphA2表达率为89.57%,Ki-67表达率为66.96%,均显著高于癌旁组织的23.78%、29.57%;EphA2和Ki-67蛋白表达与癌组织分化程度、Dukes分期、淋巴结转移均密切相关(P<0.05);随访36个月,Kaplan-Meier曲线显示EphA2、Ki-67蛋白表达阴性患者生存率明显高于表达阳性(P<0.05),Cox分析显示EphA2、Ki-67均是影响大肠癌预后的独立危险因素(P<0.001)。结论EphA2、Ki-67蛋白高表达可能在大肠癌发病、进展中参与度较高,并且可以预测患者近中期预后状况。

Objective To investigate the correlation between the expression of Eph receptor tyrosine kinase A2(EphA2)and Ki-67 protein in colorectal cancer tissues and their prognosis.Methods From July to December 2016,115 patients with colorectal cancer undergoing surgery were treated with immunohistochem-istry(SP)to detect the expression of EphA2 and Ki-67 protein in cancer tissues and adjacent tissues.Correlation between the expression of these proteins and tumor size,cancer tissue differentiation,Dukes stage,and lymph node metastasis.Kaplan-Meier curve was used to compare the relationship between EphA2,Ki-67 protein expression and cancer survival time in cancer tissues.Results The expression rate of EphA2 in cancer tissues was 89.57%,and the expression rate of Ki-67 was 66.96%,which were significantly higher than 23.78%and 29.57%of adjacent tissues.[(χ^2=45.017,P<0.001),(χ^2=32.195,P<0.001)];EphA2 and Ki-67 protein expression are closely related to the degree of cancer tissue differentiation,Dukes stage,lymph node metastasis(P<0.05);KM curve showed that patients with negative EphA2,Ki-67 protein expression were followed up for 36 months Survival rate was significantly higher than positive expression(P<0.05).Cox analysis showed that EphA2 and Ki-67 were independent risk factors affecting the prognosis of colorectal cancer(P<0.001).Conclusions The high expression of EphA2 and Ki-67 protein may be involved in the onset and progres-sion of colorectal cancer,and can predict the prognosis of patients in the near to mid-term.