FOXC1在胰腺癌中的表达及与预后的关系

Expression of FOXC1 in pancreatic cancer and its relationship with prognosis

目的分析转录因子叉头框(fork head box C1,FOXC1)表达与患者临床资料及预后的关系。方法选取上海中医药大学附属曙光医院进行胰腺根治性切除术的标本56例及配对正常癌旁胰腺组织56例,采用定量反转录聚合酶链反应(quantitative real time poly merase chain reaction,qRTPCR)和免疫组化分别检测胰腺癌组织和癌旁正常组织的FOXC1 mRNA和蛋白的表达水平,分析FOXC1表达与患者临床资料及预后的关系。结果在胰腺癌组织中FOXC1 mRNA相对表达量高于癌旁正常组织,差异有统计学意义(P<0.01)。胰腺癌组织FOXC1免疫组化阳性率71.4%高于对照组26.8%,差异有统计学意义(P<0.01)。FOXC1高表达与淋巴结转移、TNM分期、肿瘤直径存在关联(P<0.01),未发现FOXC1高表达与年龄、性别、癌症部位、分化程度存在统计学关联(P>0.05)。生存分析发现胰腺癌组中FOXC1高表达的生存时间(中位无病生存期:7个月,中位总生存期:15个月)较低表达组生存时间(中位无病生存期:18个月,中位总生存期:29个月)明显缩短,差异有意义(P<0.01),Cox多因素分析表明FOXC1表达与胰腺癌的预后相关。结论FOXC1的表达与胰腺癌的转移、TNM分型密切相关,且是胰腺癌预后的独立因素。

Objective To analyze the relationship between FOXC1 expression and clinical data and prognosis.Methods 56 cases of pancreatic radical resection and 56 cases of normal paracancerous pancreas were selected.The expressions of FOXC1 mRNA and protein in pancreatic cancer tissues and adjacent normal tissues were detected by qRT-PCR and Western blot.The relationship between FOXC1 expression and clinical data and prognosis was analyzed.Result The relative expression of FOXC1 mRNA and protein in pancreatic cancer tissues was higher than that in adjacent normal tissues(P<0.01).The positive rate of FOXC1 immunohistochemistry in pancreatic cancer tissues was 71.4%higher than that in the control group(26.8%),and the difference was statistically significant(P<0.01).The high expression of FOXC1 was associated with lymph node metastasis,TNM stage,and tumor diameter(P<0.01).There was no statistically significant correlation between age,gender,cancer location and degree of differentiation(P>0.05).Survival analysis found that DFS(7)and OS(15)with high expression of FOXC1 in pancreatic cancer group were lower than DFS(18)and OS(29)in low expression group,respectively,and the difference was significant(P<0.01).COX-Analysis showed that FOXC1 expression is associated with prognosis of pancreatic cancer.Conclusion The expression of FOXC1 is closely related to pancreatic cancer metastasis and TNM analysis,and is an independent factor in the prognosis of pancreatic cancer.