翡翠贻贝降血压肽酶解制备工艺研究

Study on the Enzymatic Hydrolysis of Antihypertensive Peptide from Perna viridis

[目的]研究翡翠贻贝蛋白酶解的工艺条件,为后期开发保健食品和药品提供基础。[方法]以ACE抑制率为指标,在单因素的基础上通过正交试验研究温度、pH、料液比和酶与底物的质量比确定酶解工艺。最后以ACE抑制率和水解率2个指标研究酶解时间。[结果]最佳酶解工艺为:碱性蛋白酶,酶解温度为50℃,酶解pH为9.5,料液比为1∶4(g∶mL),酶与底物质量比为1%,酶解时间为2 h。[结论]碱性蛋白酶可以酶解翡翠贻贝得到翡翠贻贝降血压肽,此酶解方法简单、可行。

[Objective]To study the conditions of enzymatic hydrolysis of Perna viridis,which provided a basis for the later development of medicine and health food.[Method]The temperature,pH,solid-liquid ratio,mass ratio of enzyme to substrate on enzymatic hydrolysis process was studied to obtain enzymolysis technology by orthogonal experiment on the basis of single factor experiment.Finally,the time of enzymatic hydrolysis was studied by monitoring the ACE inhibition rate and hydrolysis rate.[Result]The optimal enzymatic conditions of alkaline proteade were the temperature 50℃,pH 9.5,solid-liquid ratio 1∶4(g∶mL),mass ratio of enzyme to substrate 1%,emzymolysis time was 2 h.[Conclusion]The alkaline protease can hydrolyze Perna viridis to obtain the antihypertensive peptide of Perna viridis.This method is simple and feasible.