人初始和记忆CD4^+T细胞信使RNA基因芯片检测结果分析

Analysis of the messenger RNA from human naive and memory CD4^+ T cells by gene chips

目的探讨健康成人外周血初始和记忆CD4^+T细胞静息状态下表面分子、趋化因子受体、细胞因子和转录因子mRNA表达的差异。方法抽取健康成年人外周血,分离PBMC,染色后流式分选出CD45RO-的初始和CD45RO+的记忆CD4^+T细胞,裂解细胞,进行mRNA表达谱芯片检测。结果相比初始T细胞,静息状态下记忆CD4^+T细胞表达高水平的表面分子CTLA-4、PD-1、FAS、CD25,趋化因子受体CCR4、CCR6、CXCR3、CXCR5,细胞因子IFN-γ、TNF-α、IL-17和转录因子T-bet、EOMES、STAT4、GATA3和RORγt,并表达低水平的表面分子CD62L、CCR7、ICAM-I、CD40L,细胞因子IL-1β和转录因子NF-κB。结论静息状态下,与初始CD4^+T细胞相比,记忆CD4^+T细胞高表达某些活化分子、细胞因子、转录因子mRNA,可能是记忆CD4^+T细胞发生快速免疫应答的关键因素。

This study was designed to investigate the difference in mRNA expression of surface molecules,chemokine receptors, cytokines and transcription factors between na?ve and memory CD4^+ T cells from peripheral blood of healthy adults. Peripheral blood of healthy adults were collected to isolate PBMCs, and then, CD45 ROnaive and CD45 RO+ memory CD4^+ T cells were sorted from PBMCs by flow cytometry. The sorted cells were lysed and the detection of mRNA expression was performed. Data showed that compared to naive CD4^+ T cells, memory CD4^+ T cells expressed higher levels of surface molecules(CTLA-4, PD-1, FAS, CD25), chemokine receptors(CCR4, CCR6, CXCR3, CXCR5), cytokines(IFN-γ, TNF-α, IL-17) and transcription factors T-bet, EOMES,STAT4, GATA3 as well as RORγt. In addition, memory CD4^+ T cells expressed lower levels of surface molecules(CD62 L, CCR7, ICAM-I, CD40 L), cytokine IL-1β and transcription factor NF-κB. Taken together, the higher expression of mRNA by memory CD4^+ T cells than naive CD4^+ T cells on the activation molecules, cytokines and transcriptional factors in resting status indicate the faster immune response of memory CD4^+ T cells than naive CD4^+ T cells.