响应面优化低共熔溶剂提取乌龙茶多糖的研究

Optimization of Deep Eutectic Solvents Extraction of Polysaccharides from Oolong Tea by Response Surface Methodology

采用绿色环保高效的溶剂提取天然活性成分技术是茶资源综合利用的主攻方向之一。根据茶多糖的特性,设计出6种绿色环保高效的低共熔溶剂,利用曲面响应法优化乌龙茶多糖提取技术。结果表明,由甜菜碱和1,3-丁二醇组成的低共熔溶剂最适合茶多糖的提取。经响应曲面法优化后,茶多糖的最佳提取条件为提取时间81 min,提取温度61℃,含水率84%,固液比1∶20(g/mL),茶多糖的得率可达到6.91%。与常规水提技术相比,采用该技术提取的茶多糖得率、DPPH自由基抗氧化能力和羟自由基抗氧化能力分别提高了20.22%、53.79%、32.65%。因此,低共熔溶剂提取的茶多糖具有较强的抗氧化活性,可以为今后更深入的研究和开发提供科学依据。

The use of green and efficient solvent extraction of natural active ingredients is one of the main directions of comprehensive utilization of tea resources.Based on the characteristics of tea polysaccharides,six green environmentally-friendly and high-efficiency eutectic solvents were designed.The surface response method was used to optimize the extraction technology of oolong tea polysaccharides.The results showed that the deep eutectic solvent consisting of betaine and 1,3-butanediol was most suitable for the extraction of tea polysaccharides.After optimization with the response surface methodology,the optimal extraction conditions for tea polysaccharides were extraction time 81 min,extraction temperature 61℃,water content 84%,solid-liquid ratio 1∶20(g/mL),and the yield of tea polysaccharides could reach 6.91%.Compared with the conventional water extraction technology,the tea polysaccharide yield,DPPH antioxidant capacity and hydroxyl radical antioxidant capacity extracted by this technology were increased by 20.22%,53.79% and 32.65%,respectively.Therefore,the eutectic solvent extracted tea polysaccharide had strong antioxidant activity and could provide scientific basis for further research and development in the future.