宣木瓜抑菌特性及其饮料的活性成分研究

Study on the Antibacterial Properties of Xuan Mugua and the Properties of Its Beverage

采用平板打孔法测定宣木瓜不同极性成分对食品常见腐败菌大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑菌活性。并探讨利用乳酸菌发酵后的宣木瓜制作的低涩饮料在冷藏过程中其内部多糖、三萜、黄酮及自由基清除能力的变化。结果表明:宣木瓜中的抑菌活性成分主要分布在乙酸乙酯和正丁醇萃取层。宣木瓜饮料具有宣木瓜的有效活性成分和淡淡清香。饮品内的多糖和总酚含量在冷藏90 d后达到最小值分别为0.703 mg葡萄糖当量/mL饮料,1.101 mg儿茶素当量/mL饮料。三萜和黄酮则相反,达到最大值,分别为0.322 mg熊果酸当量/mL饮料,0.095 mg槲皮素当量/mL饮料。DPPH自由基清除能力随着冷藏时间而逐渐减小,ABTS自由基清除能力则出现先减小后增大的趋势。宣木瓜饮料具有抗氧化活性功能,平肝舒筋,和胃化湿,提高人体免疫力。

The antibacterial activity of Mugua different extract layers were investigated by the plate method of drilling hole,taking food spoilage bacteria Escherichia coli,Staphylocaccus aureus,Bacillus subtilis as the tested bacteria.A beverage was prepared by lactic acid fermentation Mugua,which were reduced sour and astringent feel.The change of polysaccharide,triterpene,flavonoids and radical scavenging abilities of beverage during the cold storage were evaluated.The results showed that ethyl acetate and n-butyl alcohol fractions of Mugua exhibited strong antibacterial activities.Mugua beverage containing active components of Xuan Mugua and light faint scent.When beverage was storaged in fridge for 90 days,total polysaccharide and total phenolics of beverage was decreased to 0.703 mg glucose equivalent/mL and 1.101 mg quercetin equivalent/mL.On the contrary,contents of triterpene and flavonoids increased to 0.322 mg ursolic acid equivalent/mL and 0.095 mg quercetin equivalent/mL.DPPH radical scavenging ability decreased gradually with the storage time,while ABTS radical scavenging ability first decreased and then increased.Xuan Mugua beverage has antioxidant activity,smooth liver muscle,and stomach moisture,improve human immunity.