海绵共附生放线菌的分离鉴定与抑菌活性分析

Isolation,Identification and Antibacterial Activity of Actinomycetes Associated with Marine Sponge

为了探究海南海绵共附生放线菌资源的多样性及潜在的药用价值,采用4种分离培养基分离海南文昌海域海绵的共附生放线菌,利用16S rRNA序列分析对分离的放线菌进行鉴定,采用滤纸片扩散法对分离放线菌的发酵产物进行抗菌活性分析。结果表明,从4种海绵中共获得50株放线菌,所分离菌株归属于5个亚目、6个科和7个属,包括链霉菌属、小单孢菌属、红球菌属、糖多孢菌、栖白蚁菌属、分枝杆菌属和Krasilnikoviella属,其中Krasilnikoviella为首次从海绵中分离得到,4株海洋放线菌为潜在的新物种。抗菌活性测试显示48%的分离菌株呈现抗细菌活性,24%的分离菌株呈现抗植物丝状病原真菌活性。本研究结果初步揭示了海南文昌海域海绵共附生放线菌的可培养物种多样性及其发酵产物的抑菌活性。

To investigate microbial diversity and potential pharmaceutical value of actinomycetes associated with marine sponges from Hainan actinomycetes associated with marine sponges from Wenchang sea area in Hainan were collected,isolated and identified,and their anti-microbial activities were analyzed.Four media were used to isolate and purify the actinomycetes from the marine sponges,and the isolates were identified according to their 16S rRNA sequences.the antibacterial activities of their fermentated products were tested using standard disk diffusion.Results showed that a total of 50 strains of actinomycetes were isolated from 4 types marine sponges.The isolates belonged to 7 genera within 6 families,5 suborders of Actinobacteria,and contained Streptomyces,micromonospora,Rhodococcus,Saccharopolyspora,Isoptericola,Mycobacterium and Krasilnikoviella,among which Krasilnikoviella was first recorded isolated from marine sponges.Four isolates were potential novel species.The antimicrobial test showed that 48%of the total isolates displayed antibacterial activity while 24%inhibited plant filamentous pathogenic fungi.The diversity and antimicrobial activity of the fermentated products of the actinomycetes associated with marine sponges collected from Wenchang sea area in Hainan were preliminarily revealed.