大羽藓形态学性状及挥发性成分的GC-MS分析

Morphology of Thuidium cymbifolium and GC-MS Analysis of Its Volatile Compounds

为探究传统中药大羽藓的形态学特征及挥发性化学成分,以海南黎母山的大羽藓(Thuidium cymbifolium (Dozy et Molk.) Dozy et Molk.)为研究材料,对其形态性状进行了描述,比较了该种与4个近缘种或变种的形态差异,并采用石油醚快速溶剂萃取技术结合GC-MS法对其挥发性物质进行萃取和化学成分分析。共鉴定出31种挥发性成分,占总组分的91.1%,主要成分类型包括酯类(25.48%),萜类(22.64%),酮类(20.64%)及甾醇类(10.61%);主要成分为丁酸丁酯(18.96%)、4-庚酮(10.90%)、角鲨烯(10.75%)和3-甲基-4-庚酮(9.74%)。

Thuidium cymbifolium,a traditional medicine,was collected from Limu Mountain in Hainan to observe its morphology and analyze its volatile composition.Its morphology was compared with that of its 4 relatives and varieties to find out their morphological difference,and its volatile compounds was extracted by using accelerated solvent extraction(ASE)with petroleum ether as solvent and then analyzed by using gas chromatography-mass spectrometry(GC-MS).The analysis showed that the volatile compounds of T.cymbifolium were identified to contain 31 constituents,accounting for 91.1%of the total chemical composition.The main constituents were esters(25.48%),terpenes(22.64%),ketones(20.64%)and sterols(10.61%),including butyl butyrate(18.96%),4-heptanone(10.90%),squalene(10.75%)and 4-heptanone,3-methyl(9.74%).